초록

An immunoassay for analytes such as antigens or haptens, which utilizes covalent hybrid antibodies to modulate the activity of indicators. The hybrid antibody has binding sites for the analyte and the indicator. Final activity of the indicator is proportional to analyte concentration.

대표청구항 ▼

A homogeneous immunoassay for the measurement of analyte comprising the steps of incubating sequentially or simultaneously: (A) a substantially pure covalent hybrid antibody consisting essentially of two different heavy chain-light chain half-molecules, wherein the first of said half-molecules provi

A homogeneous immunoassay for the measurement of analyte comprising the steps of incubating sequentially or simultaneously: (A) a substantially pure covalent hybrid antibody consisting essentially of two different heavy chain-light chain half-molecules, wherein the first of said half-molecules provides a binding site for a first antigen and the second of said half-molecules provides a chemically different binding site for the first or a second antigen; wherein said half-molecules are bonded to each other through disulfide linkage; (B) a biological sample containing analyte; (C) an indicator capable of being bound by said hybrid antibody; and (D) reagents for signal-generating reaction.