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특허 상세정보

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Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) G01N-033/54    G01N-033/68    G01N-027/26   
미국특허분류(USC) 436/506 ; 204/403 ; 435/7 ; 436/516
출원번호 US-0261217 (1981-04-20)
우선권정보 CA-0348033 (1980-03-20)
국제출원번호 PCT/EP80/00018 (1980-03-18)
§371/§102 date 19810420 (19810420)
국제공개번호 WO-8102790 (1981-10-01)
발명자 / 주소
  • Gordon Julian (Arlesheim CHX) Staehelin Theophil (Arlesheim CHX) Towbin Harry (Allschwil CHX)
출원인 / 주소
  • Ciba-Geigy Corporation (Ardsley NY 02)
인용정보 피인용 횟수 : 94  인용 특허 : 0
초록
▼

Solid supports for proteins consisting of nitrocellulose sheets containing a replica of an electropherogram of proteins as obtained by electrophoretic separation in a gel. Faithful replica of such electropherograms on the nitrocellulose support can be obtained by contacting the gel with a nitrocellulose sheet and applying an electric field perpendicular to the plane of the gel causing an electrophoretic migration of the proteins toward the nitrocellulose sheet where the proteins are adsorbed. Various analytical problems and especially immuno-assays, for ...

대표
청구항
▼

A solid support for proteins consisting of a porous nitrocellulose sheet containing a replica of an electropherogram of proteins in a gel wherein the replica is obtained by electrophoretic transfer of said electropherogram from the gel. A process for obtaining a solid support for protein consisting of a porous nitrocellulose sheet containing a replica of an electropherogram of proteins in a gel, comprising transferring the electropherogram from the gel to the nitrocellulose sheet by electrophoresis. An immunoassay for the identification or detection of p...

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