초록 ▼

Droplet counter-current chromatography is utilized to isolate and purify 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol, and the diastereoisomer 4a

Droplet counter-current chromatography is utilized to isolate and purify 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol, and the diastereoisomer 4a

대표청구항 ▼

A method for isolating 12-O-tetradecanoylphorbol-13-acetate(TPA) in high purity from croton oil comprising the steps of (a) extracting the croton oil with an aqueous alcohol solution to obtain a crude TPA extract; (b) purifying the crude TPA extract by droplet counter-current chromatography(DCCC) wh

A method for isolating 12-O-tetradecanoylphorbol-13-acetate(TPA) in high purity from croton oil comprising the steps of (a) extracting the croton oil with an aqueous alcohol solution to obtain a crude TPA extract; (b) purifying the crude TPA extract by droplet counter-current chromatography(DCCC) wherein the mobile and stationary phases are derived from an equilibrated mixture of hexane-diethylether-n-propanol-ethanol-water, the separated upper layer of which comprises the stationary phase and the bottom layer of which the mobile phase, and the composition of which mixture is such that the distribution coefficient for TPA, as defined by the ration of its concentration in the stationary phase divided by that in the mobile phase, lies in the range of from about 3.0 to about 12.0; and (c) further purifying the TPA by liquid-column chromatography followed by thin-layer chromatography.