초록 ▼

An immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises contacting the fluid with at least one first entity selected from a group consisting of an antibody (Ab) to the Ag, a soluble, labeled antibody (L-Aba) to the Ag, and an antibody (Abb) to

An immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises contacting the fluid with at least one first entity selected from a group consisting of an antibody (Ab) to the Ag, a soluble, labeled antibody (L-Aba) to the Ag, and an antibody (Abb) to the Ag bound to a solid support (SC). The immunoassay is characterized in that the fluid is contacted with at least one additional entity selected from a group consisting of at least one different type of soluble, labeled antibody (L-Abc) to the Ag, at least one different type of antibody (Abd) bound to a solid carrier (SC1), and at least one different type of antibody (Abe) to the Ag. The SC1 is selected from a group consisting of SC, at least one different solid carrier (SC2), and mixtures thereof (SC and SC2). Each type of L-Abc, Abd-SC1, and Abe has a lower average affinity constant (K) for Ag than each respective K of L-Aba, Abb-SC and Ab e; and the additional entity is present in an amount sufficient to avoid a hook effect. Also, a reagent of the type comprising at least one first entity selected from a group consisting of L-Aba, Abb-SC, and Ab. The reagent is characterized in that it further comprises at least one additional entity selected from a group consisting of at least one different type of L-Abc and at least one different type of Abd-SC1, and at least one different type of Abe. Ab, L-Aba, Abb-SC, L-Abc, Abd-SC1, and Abe are as defined above.

대표청구항 ▼

A one-step sandwich immunoassay for an antigenic substance (Ag) in a fluid, said immunoassay being of the type comprising: (a) contacting said fluid with (i) a soluble, labeled antibody (L-Aba) to said Ag and (ii) an antibody (Abb) to said Ag bound to a solid carrier (SC) to form an insoluble comple

A one-step sandwich immunoassay for an antigenic substance (Ag) in a fluid, said immunoassay being of the type comprising: (a) contacting said fluid with (i) a soluble, labeled antibody (L-Aba) to said Ag and (ii) an antibody (Abb) to said Ag bound to a solid carrier (SC) to form an insoluble complex (L-Aba-Ag-Abb-SC); (b) separating said L-Aba-Ag-Abb-SC from said fluid and unreacted L-Aba; and (c) measuring either the amount of L-Aba associated with said L-Aba-Ag-Abb-SC or the amount of unreacted L-Aba; characterized in that in step (a) said fluid is also contacted with at least one additional entity selected from the group consisting of at least one different type of soluble, labeled antibody (L-Abc) to said Ag and at least one different type of antibody (Abd) to said Ag bound to a solid carrier (SC1); wherein: (i) each type of L-Abc and Abd-SC1 has a lower average affinity constant (K) for said Ag than each respective K of L-Aba and Abb-SC; (ii) said additional entity is present in an amount sufficient to avoid a hook effect; and (iii) said SC1 is selected from the group consisting of said SC, at least one different solid carrier (SC2), and mixtures thereof (SC and SC2). A reagent of the type comprising: (a) a soluble, labeled antibody (L-Aba) to an antigenic substance (Ag); and (b) an antibody (Abb) to said Ag bound to a solid carrier (SC); characterized in that said reagent further comprises at least one additional entity selected from the group consisting of at least one different type of soluble, labeled antibody (L-Abc) to said Ag and at least one different type of antibody (Abd) to said Ag bound to a solid carrier (SC1); wherein: (i) each type of L-Abc and Abd-SC1 has a lower average affinity constant (K) for said Ag than each respective K of L-Aba and Abb-SC; (ii) said additional entity is present in an amount sufficient to avoid a hook effect when said reagent is employed in an immunoassay; and (iii) said SC1 is selected from the group consisting of said SC, at least one different solid carrier (SC2), and mixtures thereof (SC and SC2).