초록 ▼

There is disclosed an immunoassay comprising carrying out an immunological reaction by contacting a fluid sample with an element for immunoassay, having at least three layers of a first detecting layer, a blocking layer and a second detecting layer which are successively laminated, the element conta

There is disclosed an immunoassay comprising carrying out an immunological reaction by contacting a fluid sample with an element for immunoassay, having at least three layers of a first detecting layer, a blocking layer and a second detecting layer which are successively laminated, the element containing a substance capable of binding specifically to an immunologically active substance in a fluid sample only in either one of the first and the second detecting layers, and then measuring the detected quantities corresponding to the immunological reaction quantities from both of the first and the second detecting layers. The immunoassay by the use of the immunological analytical element according to this invention improves the precision immunoassay by measuring both of Bound and Free.

대표청구항 ▼

An immunoassay method for measuring an immunological antigen in a fluid sample, which comprises: contacting said fluid sample with a first detecting layer of an immunological analytical element in the presence of a labeled antigen, said element comprising sequentially the first detecting layer, a bl

An immunoassay method for measuring an immunological antigen in a fluid sample, which comprises: contacting said fluid sample with a first detecting layer of an immunological analytical element in the presence of a labeled antigen, said element comprising sequentially the first detecting layer, a blocking layer, a second detecting layer and a substrate and at least one of said first and second detecting layers contains an antibody capable of binding specifically to said antigen and labeled antigen to carry out a competitive reaction of said antigen and said labeled antigen with said antibody; and separately measuring the amount of the unreacted labeled antigen and the amount of binding complex of said labeled antigen and said antibody of both said first and said second detecting layers to determine the amount of said antigen in said fluid sample.