초록 ▼

An antigen/antibody precipitate is obtained, using monoclonal antibodies, the monoclonal antibodies (samples I or II or III or IV) being selected so as to be specific to two distinct antigenic binding sites (L or C 2 or C 3) on a protein (IgG) in a sample under test. The proportions of sub-populatio

An antigen/antibody precipitate is obtained, using monoclonal antibodies, the monoclonal antibodies (samples I or II or III or IV) being selected so as to be specific to two distinct antigenic binding sites (L or C 2 or C 3) on a protein (IgG) in a sample under test. The proportions of sub-populations of immunoglobulins (IgG kappa, IgG lambda) in a sample is determined by reacting the sample with a combination of antibodies (II and IV) both of which are specific to the heavy chains (H) of both sub-populations (IgG kappa, IgG lambda) and reacting the sample with an antibody combination (I and II) specific to said heavy chain (H) and to an antigenic determinant expressed by only one (IgG kappa) of the sub-populations.

대표청구항 ▼

A method for quantification of a sub-population of an immunoglobulin having first and second sub-populations, said method comprising the steps of: (a) reacting a first sample containing said immunoglobulin with first and second monoclonal antibody preparations which specifically bind to respective d

A method for quantification of a sub-population of an immunoglobulin having first and second sub-populations, said method comprising the steps of: (a) reacting a first sample containing said immunoglobulin with first and second monoclonal antibody preparations which specifically bind to respective distinct antigenic sites on the macromolecule of said immunoglobulin, such that binding of one monoclonal antibody to its specific antigenic site does not intefere with the binding of the other antibody to its specific antigenic site, both of said sites being common to both of said first and second sub-populations, to obtain a first antigen-antibody precipitate; (b) reacting a second sample identical to said first sample with said first preparation and with a third monoclonal antibody preparation which specifically binds to an antigenic site expressed by only one of said first and second sub-populations, to obtain a second antigen-antibody precipitate; and (c) determining from said second antigen-antibody precipitate the quantity of said first sub-population in the sample.