초록 ▼

Disclosed herein is an assay useful for the determination of an analyte in whole blood. In particular, this assay is useful for the quantitative determination of peroxide-generating analytes, such as glucose or cholesterol, in whole blood. This assay utilizes a multizone element consisting essential

Disclosed herein is an assay useful for the determination of an analyte in whole blood. In particular, this assay is useful for the quantitative determination of peroxide-generating analytes, such as glucose or cholesterol, in whole blood. This assay utilizes a multizone element consisting essentially of a support having thereon, in order and in fluid contact, a registration zone and a reagent/spreading zone. The reagent/spreading zone has a void volume and average pore size effective to accommodate whole blood, and contains an interactive composition necessary for the analysis. Such composition is capable of providing, upon interaction with the analyte, a dye which can be spectrophotometrically detected at a wavelength greater than about 600 nm.

대표청구항 ▼

A method for the determination of an analyte other than hemoglobin in whole blood comprising the steps of: (A) physically contacting a sample of whole blood containing an analyte other than hemoglobin and a multizone element, said element consisting essentially of support having thereon, in order an

A method for the determination of an analyte other than hemoglobin in whole blood comprising the steps of: (A) physically contacting a sample of whole blood containing an analyte other than hemoglobin and a multizone element, said element consisting essentially of support having thereon, in order and in fluid contact, a registration zone and a reagent/spreading zone, provided said element does not contain a radiation-blocking zone, said reagent/spreading zone having a void volume and average pore size effective to accommodate whole blood, an containing an interactive composition capable of providing, upon interaction with said analyte, a dye which can be spectrophotometrically detected at a wavelength greater than about 600 nm; and (B) detecting said dye at a wavelength greater than about 600 nm.