초록 ▼

A process is disclosed for recovering renatured polypeptides and proteins (collectively “polypeptides”) from solution thereof and/or of their S-sulfonate derivatives (collectively “solution of polypeptides”) in denaturants comprising: introducing said solution into a renaturant chamber (compartment,

A process is disclosed for recovering renatured polypeptides and proteins (collectively “polypeptides”) from solution thereof and/or of their S-sulfonate derivatives (collectively “solution of polypeptides”) in denaturants comprising: introducing said solution into a renaturant chamber (compartment, cell, space) of an electrodialysis (“ED”) apparatus, said chamber bounded by membranes each of which is substantially permeable to low molecular weight singly charged ions of at least one charge sign and substantially impermeable to said polypeptides and S-sulfonate derivatives, said membranes separating said renaturant chamber from juxtaposed rinsing chambers on each side of the renaturant chamber; introducing rinsing solutions into said juxtaposed rinsing chambers, said rinsing solutions synergetically with said membranes facilitating a decrease in the chaotropism of said denaturant in said renaturant chamber when a direct electric current is imposed through said renaturant and rinsing chambers in series; applying a direct electric current (which may have a substantial alternating current component) in series through said juxtaposed renaturant and rinsing chambers in a direction which causes a decrease in the chaotropism of said denaturant in said renaturant chamber thereby decreasing the chaotropism of said denaturant in said solution of polypeptide and/or S-sulfonate derivatives to a chaotropic level which permits substantial refolding of said polypeptides and/or S-sulfonate derivatives, which level is not less than that which results in substantial precipitation of said polypeptide and/or S-sulfonate derivative out of said solution; maintaining the pH of said solution of polypeptide and/or S-sulfonate derivate at pH\s in the range of from about 4 to about 10.

대표청구항 ▼

A process for recovering at least one substantially renatured polypeptide from a solution of said polypeptide and/or its S-sulfonate derivative reversibly denatured in aqueous denaturant, said process comprising: (a) introducing said solution of said polypeptide and/or its S-sulfonate derivative int

A process for recovering at least one substantially renatured polypeptide from a solution of said polypeptide and/or its S-sulfonate derivative reversibly denatured in aqueous denaturant, said process comprising: (a) introducing said solution of said polypeptide and/or its S-sulfonate derivative into at least one renaturant compartment of an electrodialysis apparatus, said compartment defined by membranes each of which is substantially permeable to low molecular weight singly charged ions of at least one charge sign and substantially impermeable to said polypeptides and S-sulfonate derivatives, said membranes also defining rinsing compartments juxtaposed on each side of said renaturant compartment; (b) introducing second aqueous solutions into said juxtaposed rinsing compartments, said second solutions adapted in combination with said membranes to permit reduction of the chaotropy of said denaturant in said renaturant compartment upon the application of a direct electric current in at least one of the possible directions through said renaturant compartment and said juxtaposed rinsing compartments in series; passing a substantially direct electric current in series through said renaturant compartment and said juxtaposed rinsing compartments in a direction to cause reduction of the chaotropy of said denaturant in said renaturant compartment thereby reducing the chaotropy of said denaturant in said solution of polypeptide and/or S-sulfonate derivative to a level characterized by its ability to permit at least partial refolding of such polypeptide and/or S-sulfonate derivative which level is not less than that which would result in substantial precipitation of said polypeptide and/or its S-sulfonate derivatives out of said solution at pH\s in the range of from about 4 to about 10 , which pH\s do not substantially decrease the solubility of said polypeptide and/or S-sulfonate derivative at said chaotropy level; (d) maintaining the pH of said solution of polypeptide and/or S-sulfonate derivative during electrodialysis at pH\s in the range of from about 4 to about 10 which pH\s are characterized by not substantially decreasing the solubility of said polypeptide and/or S-sulfonate derivatives at said reduced chaotropy level; and (e) removing from said renaturant compartment a solution of said polypeptide and/or S-sulfonate derivative having a substantially reduced chaotropy level.