초록 ▼

Cells are identified electrophoretically through isoenzyme analysis. The use of standard and control cells allows semi-skilled workers to achieve reproducible results. A coding sequence is assigned to the cels to be identified using a grid to interpret the electrophoretic separations observed on a s

Cells are identified electrophoretically through isoenzyme analysis. The use of standard and control cells allows semi-skilled workers to achieve reproducible results. A coding sequence is assigned to the cels to be identified using a grid to interpret the electrophoretic separations observed on a series of electrophoretic films, each film being developed for a particular isoenzyme. Comparison of the coding sequence with a compendium of coding sequences for known cells identifies the cells to be identified.

대표청구항 ▼

An electrophoretic method for identifying cells comprising the steps of: (a) simultaneously performing on an electrophoretic film, multiple electrophoretic separations on a sample from a control, a standard population of cells, and at least one sample from an unknown population of cells each of the

An electrophoretic method for identifying cells comprising the steps of: (a) simultaneously performing on an electrophoretic film, multiple electrophoretic separations on a sample from a control, a standard population of cells, and at least one sample from an unknown population of cells each of the samples containing a least some electrophoretically separable substances which are characteristic of all the cells; (b) developing the electrophoretic separations of control, standard, and unknown cells with regard to an electrophoretically separable, characteristic substance; (c) comparing the developed separations for the control and standard samples to determine the range of separation for the characteristic substance under the conditions used; and (d) based on the range of separation so determined, comparing the separations for the unknown and standard samples to identify the unknown cells.