[특허]Enzyme-free diagnostic binding reagents

[미국특허] Enzyme-free diagnostic binding reagents 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12Q-001/68 G01N-033/566 G01N-033/53 G01N-033/542
출원번호	US-0547232 (1983-10-31)
발명자 / 주소	Klotz Irving M. (2515 Pioneer Rd. Evanston IL 60201)
인용정보	피인용 횟수 : 10 인용 특허 : 7

초록 ▼

An enzyme-free diagnostic reagent, methods of its use and diagnostic systems containing that reagent are disclosed. The enzyme-free diagnostic reagent comprises an enzyme-free catalyst coupled by a linking group to a first binding agent, binds in aqueous medium to a second binding moiety to form a binding complex, and indicates the amount of second binding moiety present in the complex by means of its catalytic reactivity with co-reactant molecules.

대표청구항 ▼

A method of carrying out a binding assay comprising the steps of (a) providing an enzyme-free diagnostic reagent for determining the presence of an entity to be assayed in a homogeneous assay comprising an enzyme-free hydrolytic catalyst coupled by a linking group to a first binding agent, said catalyst having a molecular weight together with the linking group of about 75 to about 2000 daltons, wherein said catalyst catalyzes a hydrolytic chemical reaction with successive preselected hydrolyzable co-reactant molecules when said co-reactant molecules and said catalyst are admixed. in homogeneous solution and said catalyst is coupled to said first binding agent as said diagnostic reagent; said diagnostic reagent specifically binding in aqueous medium to a second binding moiety to form a binding complex, said second binding moiety being (a) the entity to be assayed in a direct assay, or (b) an entitity needed for the assay in an indirect assay, said diagnostic reagent indicating the amount of said second binding moiety present in said binding complex by means of the catalytic reactivity of said catalyst with said co-reactant molecules, said catalytic reactivity being changed when said binding complex is formed; (b) providing a first aqueous composition containing said second binding moiety; (c) admixing in an aqueous medium a known, predetermined amount of said diagnostic reagent and a known, predetermined amount of said first aqueous composition to form a binding complex between said diagnostic reagent and said second binding moiety; (d) admixing a plurality of hydrolyzable co-reactant molecules with the admixture of step (c); and (e) measuring the reaction between said enzyme-free catalyst and said co-reactant molecules.

이 특허에 인용된 특허 (7) 인용/피인용 타임라인 분석

Ullman Edwin F. (Atherton CA), Catalyst mediated competitive protein binding assay.
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Zuk Robert F. (San Francisco CA) Gibbons Ian (Menlo Park CA) Rowley Gerald L. (Cupertino CA) Ullman Edwin F. (Atherton CA), Double antibody for enhanced sensitivity in immunoassay.
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Ullman Edwin F. (Atherton CA) Schwarzberg Moshe (Palo Alto CA), Fluorescence quenching with immunological pairs in immunoassays.
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Forgione Peter S. (Stamford CT) Henderson ; Jr. William A. (Stamford CT), Heterogeneous chemiluminescent immunoassays utilizing metallo porphyrin tag.
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Boguslaski, Robert C.; Carrico, Robert J., Homogeneous chemiluminescent specific binding assay.
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Dreesman Gordon R. (Houston TX) Kendall Cynthia E. (Colombia MO), Immunoassay employing monoclonal herpes simplex antibody and biotin-avidin detection system.
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Hodgins Leonard T. (New York NY) Finlay Thomas H. (Ridgewood NJ) Johnson Alan J. (New York NY), Preparation of trichloro-s-triazine activated supports for coupling ligands.
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이 특허를 인용한 특허 (10) 인용/피인용 타임라인 분석

Hiatt Andrew C. ; Rose Floyd, 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds.
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Hiatt Andrew C. ; Rose Floyd, 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds.
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Southern, Edwin, Analysing polynucleotide sequences.
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Southern, Edwin, Analysing polynucleotide sequences.
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Hiatt Andrew C. ; Rose Floyd, Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides.
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Hiatt Andrew C. ; Rose Floyd, Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides.
상세보기
Hiatt Andrew C. ; Rose Floyd, Enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides.
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Lazar James G. ; Taub Floyd E., Macromolecular conjugate made using unsaturated aldehydes.
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Schiff Robert (Caldwell NJ) Lehnus Gary (Blairestown NJ) Wasserman Charles J. (Muskego WI) Garlock Henrietta M. (Oconomowoc WI), Polymer coated solid matrices and use in immunoassays.
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Adams Craig W. (King of Prussia PA) Leary Jeffry J. (Brea CA) Rosenberg Martin (Malvern PA), Polynucleotide hybridization probes.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Enzyme-free diagnostic binding reagents 원문보기