초록 ▼

A process of immunoassay of a selected substance in a liquid sample containing one or more other non-selected substances, each of these substances being comprised of at least two structurally different chains, the process comprising among others addition of a dissociating agent, such as for example

A process of immunoassay of a selected substance in a liquid sample containing one or more other non-selected substances, each of these substances being comprised of at least two structurally different chains, the process comprising among others addition of a dissociating agent, such as for example a proteolytic enzyme, to the sample, mixing of the so-obtained liquid, inactivation of the dissociating agent, addition of antiserums, and determination of the amount of this selected substance in the liquid sample.

대표청구항 ▼

A process of immunoassay of a selected substance in a liquid sample containing at least one other substance which can interfere with the assay of said selected substance, said selected substance and said other substance each being composed of at least two structurally different fragments, a first fr

A process of immunoassay of a selected substance in a liquid sample containing at least one other substance which can interfere with the assay of said selected substance, said selected substance and said other substance each being composed of at least two structurally different fragments, a first fragment of said selected substance being immunologically the same as a first fragment of said other substance, a second fragment of said selected substance being immunologically distinct from any fragment of said other substance, wherein said process comprises the steps of: adding a dissociating agent selected from the group consisting of proteolytic agents, agents capable of breaking disulfide bridges, chaotropic agents, and mixtures thereof, to said liquid sample in order to split said selected substance into at least two fragments, said other substance being also split into at least two fragments, inactivating said dissociating agent, adding to said sample liquid an antiserum directed against said first fragment of said selected substance and said first fragment of said other substance , adding an antiserum directed against the immunologically distinct fragment of said selected substance to said liquid sample, and determining the amount of said selected substance in said liquid sample.