초록 ▼

Novel substances having carcinostatic and immunostimulating activity, which are obtained from a culture or its supernatant fluid prepared by culturing bacteria belonging to Fusobacterium genus. Said substances are useful for the treatment of cancerous diseases lower warm-blooded animals. This disclo

Novel substances having carcinostatic and immunostimulating activity, which are obtained from a culture or its supernatant fluid prepared by culturing bacteria belonging to Fusobacterium genus. Said substances are useful for the treatment of cancerous diseases lower warm-blooded animals. This disclosure concerns such substances and a process for preparing the same and a carcinostatic agent containing the same.

대표청구항 ▼

A substance TF-100 having the following properties: (a) Grayish white－light brown powder, (b) It inhibits the proliferation of mouse Ehrlich ascites tumor and Ehrlich solid tumor, and has an immunostimulating activity, (c) It is soluble in water and it is insoluble in methanol, ethanol, acetone, ben

A substance TF-100 having the following properties: (a) Grayish white－light brown powder, (b) It inhibits the proliferation of mouse Ehrlich ascites tumor and Ehrlich solid tumor, and has an immunostimulating activity, (c) It is soluble in water and it is insoluble in methanol, ethanol, acetone, benzene, chloroform, ethyl acetate and diethyl ether, (d) It has no clear melting point, begins to decompose at about 110°C., and decomposes remarkably above 200°C., (e) Its infrared absorption spectrum obtained by a KBr tablet method has absorption bands in the vicinity of 3600-3200, 2960-2930, 1670-1640, 1550, 1440-1380, 1240, 1140-1000 and 820 cm-1, (f) The ultraviolet absorption spectrum of its aqueous solution shows a strong absorption at the absorption edge, and shows an absorption peak in the vicinity of 256-260 nm, (g) When it is fractionated by gel filtration (column: 50 mmfmm, eluent: distilled water) by using Sephadex G-50 (registered trademark of Pharmacia Co., Ltd.), it has absorption bands at the elution volumes in the vicinity of the void volume－400, 430-530, 700-800, and 840-870 ml in the ultraviolet absorbance measurement at 260 nm; and at the elution volumes in the vicinity of the void volume－390, and 410-430 ml in the absorbance measurement at 620 nm by an anthrone-sulfuric acid method, (h) Its high performance liquid chromatogram (eluent: a 0.1M phosphate buffer having a pH of 7, flow rate: 0.8 ml/min., at room temperature) obtained by using TSK-GEL G3000 SW (trade name of Toyo Soda Co., Ltd., column: 7.9 mmfmm×2) has peaks in the vicinity of the solvent front, 38-60, and 65 min. in the ultraviolet absorbance measurement at 220 nm and 260 nm, (i) It is positive in Molisch reaction,, phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, indole-hydrochloric acid reaction, Lowry-Folin\s reaction, and ninhydrin reaction, (j) Elementary analysis values C: 30.6-35.7%, H: 4.2-5.2%, N: 4.2-5.2% (k) Its saccharide content measured by a phenol-sulfuric acid method is about 40.0－about 46.0% by weight in terms of glucose, and its protein content measured by Lowry-Folin\s method is about 20.0－about 23.0% by weight in terms of calf serum albumin.