[특허]Immunoassay for measuring the concentration of antigen in a sample

[미국특허] Immunoassay for measuring the concentration of antigen in a sample 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/53
출원번호	US-0867554 (1986-05-28)
우선권정보	JP-0114084 (1985-05-29)
발명자 / 주소	Nagai Keiichi (Tokyo JPX) Tokinaga Daizo (Hachioji JPX) Imai Kazumichi (Kodaira JPX) Yasuda Kenji (Tokyo JPX) Takahashi Satoshi (Kokubunji JPX) Kobayashi Teruaki (Hachioji JPX)
출원인 / 주소	Hitachi, Ltd. (Tokyo JPX 03)
인용정보	피인용 횟수 : 13 인용 특허 : 4

초록 ▼

An immunoassay comprising immobilizing antibody in a matrix for electrophoresis; immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis; either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; and measuring the concentration of antigen in the sample, characterized by using as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance, moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis, reacting the substrate with the label enzyme to convert the same into a fluorescent substance, and measuring the concentration of the fluorescent substance in the electrolyte solution.

대표청구항 ▼

An immunoassay comprising: immobilizing antibody in substantially the whole portion of a matrix for electrophoresis; immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis; either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned antibody by electrophoresis to react the same with the unreacted portion; measuring the concentration of antigen in the sample, characterized by using as a label for the labeled antibody or the labeled antigen an enzyme capable of converting a substrate into a fluorescent substance, moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis, reacting the substrate with the label enzyme to convert the same into a fluorescent substance, moving said fluorescent substance from the matrix for electrophoresis to electrolytic solution, and then measuring the concentration of the formed fluorescent substance in the electrolyte solution.

이 특허에 인용된 특허 (4) 인용/피인용 타임라인 분석

Wadsworth Charles (Goteborg SEX), Determination of immunologically active materials and system therefore.
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Gordon Julian (Arlesheim CHX) Staehelin Theophil (Arlesheim CHX) Towbin Harry (Allschwil CHX), Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays.
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Tokinaga Daizo (Hachioji JPX) Kobayashi Teruaki (Hachioji JPX) Imai Kazumichi (Kokubunji JPX), Immunoassay method.
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Lee Martin J. (Leonia NJ) Ornstein Leonard (White Plains NY), Method and apparatuses for electrophoretic immunoassay.
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이 특허를 인용한 특허 (13) 인용/피인용 타임라인 분석

Sun, Zhen Hong; Pan, Tao; Wang, Wendy; Liu, Xuanbin, Apparatus and method of using porous material for antigen separation, identification, and quantification with electrophoresis techniques.
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Rothschild, Kenneth J.; Olejnik, Jerzy; Sonar, Sanjay M., Dual markers.
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Frank, Ronald; Tegge, Werner, Immobilized substrate, separation gels and method for detecting enzymatic activity.
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Arnquist David C. ; Barnes ; III Grady ; Button Richard D. ; Dunn Chadwick M. ; East ; Jr. Richard C. ; Fritchie Patrick P. ; Galitz Charles M. ; Gardner Gregory E. ; Grandone Cass J. ; Gray Robert C, Method for determination of item of interest in a sample.
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Rothschild Kenneth J. ; Sonar Sanjay M. ; Olejnik Jerzy, Method for the detection and isolation of protein.
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Hayashi Hidechika (Yokohama JPX) Umegae Yoshihiko (Yokohama JPX) Mitsuhisa Yukio (Ebina JPX), Method of assay of enzymatic activity and apparatus therefor.
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Hayashi Hidechika,JPX ; Umegae Yoshihiko,JPX ; Mitsuhisa Yukio,JPX, Method of assay of enzymatic activity and apparatus therefor.
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Arnquist David C. ; Barnes ; III Grady ; Dunn Chadwick M. ; East ; Jr. Richard C. ; Fritchie Patrick P. ; Gardner Gregory E. ; Grandone Cass J. ; Gray Robert C. ; Holen James T. ; McCoy Jimmy D. ; Mi, Method of performing a process for determining an item of interest in a sample.
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Rothschild Kenneth J. ; Sonar Sanjay M. ; Olejnik Jerzy, Methods for the detection and isolation of biomolecules.
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Rothschild Kenneth J. ; Sonar Sanjay M. ; Olejnik Jerzy, Photocleavable agents and conjugates for the detection and isolation of biomolecules.
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Rothschild Kenneth J. ; Sonar Sanjay M. ; Olejnik Jerzy, Photocleavable agents and conjugates for the detection and isolation of biomolecules.
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Harold C. Warren ; Brian A. Snyder ; Lisa D. Sprague ; Shirley Y. Lynn ; Paul B. Contestable ; Holly L. Groth, Reduced cortisol conjugates.
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Arnquist, David C.; Barnes, III, Grady; Button, Richard D.; Dunn, Chadwick M.; East, Jr., Richard C.; Fritchie, Patrick P.; Galitz, Charles M.; Gardner, Gregory E.; Grandone, Cass J.; Gray, Robert C., Structure for determination of item of interest in a sample.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Immunoassay for measuring the concentration of antigen in a sample 원문보기