초록 ▼

An insert for a roller bottle of the type used in cell culture for vaccine manufacture is formed of a polymeric resilient material whereby it can be bent or rolled so that it can pass through the opening of the roller bottle. Once the insert is inside the bottle, it is released so that its resilient

An insert for a roller bottle of the type used in cell culture for vaccine manufacture is formed of a polymeric resilient material whereby it can be bent or rolled so that it can pass through the opening of the roller bottle. Once the insert is inside the bottle, it is released so that its resilient characteristic causes it to unbend or unroll. The insert is wider than the diameter of the roller bottle, and therefore it is urged against the interior wall of the roller bottle. Apertures and/or notches are provided in the insert to increase the turbulence of a fluid and to decrease the fluidic shear forces in the roller bottle as it is rotated in conventional operation. A significant increase in productivity is achieved because particles, which may be microcarrier beads, are maintained in suspension, and prevented from sinking to the bottom of the fluid medium where cells cannot grow. The roller bottle system is thereby transformed into a microcarrier suspension culture system.

대표청구항 ▼

A roller bottle system comprising: roller bottle means having an interior surface defining an interior volume characterized by a predetermined internal longitudinal length, a predeterminable internal width measured transverse to said predetermined internal longitudinal length, and a predetermined in

A roller bottle system comprising: roller bottle means having an interior surface defining an interior volume characterized by a predetermined internal longitudinal length, a predeterminable internal width measured transverse to said predetermined internal longitudinal length, and a predetermined internal perimeter measured transverse to said predetermined internal longitudinal length, said roller bottle means further having a closable opening through which access to said interior volume of said roller bottle means is obtained; and insert sheet means for maintaining particulate matter in suspension in a fluid during operation of said roller bottle means, said insert sheet means being formed of resilient material having a longitudinal length which is shorter than said predetermined internal longitudinal length and a width dimension which is greater than said predetermined internal width and shorter than said predetermined internal perimeter, said insert sheet means having an undeployed state during which said insert sheet means is inserted into said roller bottle means through said closable opening, and a deployed state after said insert sheet means is inserted into said roller bottle means, said insert sheet means further having: first and second longitudinal edge portions arranged along respective first and second longitudinal edges thereof, said first and second longitudinal edges being distal from one another by a distance corresponding to said width dimension, said first and second longitudinal edge portions being urged against respective portions of said interior surface of said roller bottle means during said deployed state.