초록 ▼

An automatic analysis method of and apparatus for the full automation of an enzyme reaction analysis from the pretreatment step of the reaction to the data processing step, wherein the operation of the pretreatment step is arranged to be sequentially performed on many samples with full automation by

An automatic analysis method of and apparatus for the full automation of an enzyme reaction analysis from the pretreatment step of the reaction to the data processing step, wherein the operation of the pretreatment step is arranged to be sequentially performed on many samples with full automation by the use of robots and computers to improve savings efficiency and measurement accuracy. Also, the pretreatment steps of weighing samples in many sample tubes, adding the given amount of solvent corresponding to the weighing value and placing the samples into the dissolution vessel to dissolve the sample in the solvent are adapted to be sequentially performed with full automation with the use of the robot, computer and electronic balance. Furthermore, the filtration, concentration and the injecting operations into the HPLC are automatically performed. The automatic apparatuses are coupled to each other so that the filtration, concentration, injecting operations are allowed to be sequentially performed with full automation using an on-line system.

대표청구항 ▼

An apparatus for automatically analyzing the effect of a sample on an enzyme, said apparatus comprising: weighing means for weighing a sample; dissolving solvent adding means for adding solvent to the sample; dissolving means for facilitating the dissolution of the sample in the solvent to produce a

An apparatus for automatically analyzing the effect of a sample on an enzyme, said apparatus comprising: weighing means for weighing a sample; dissolving solvent adding means for adding solvent to the sample; dissolving means for facilitating the dissolution of the sample in the solvent to produce a sample solution; dilution solvent adding means for adding solvent to the sample solution to produce a diluted sample solution; diluted sample solution pipetting means for injecting at least a portion of the diluted sample solution into a reaction tube; enzyme adding means for adding an enzyme into the reaction tube into which the diluted sample solution is injected by said diluted sample solution pipetting means; substrate adding means for adding a substrate of the enzyme into the reaction tube to obtain a reaction mixture in which an enzyme reaction takes place; a preservation vessel for preserving the reaction mixture; moving means for moving the reaction tube into said preservation vessel to preserve the reaction mixture; filtering means for filtering the reaction mixture as preserved by the preservation vessel to produce a filtrate of the reaction mixture; concentrating means for concentrating the filtrate to produce a concentrated filtrate of the reaction mixture; analysis means for producing data based on an analysis of the concentrated filtrate of the reaction mixture; filtrate injecting means for injecting the concentrated filtrate of the reaction mixture into the analysis means to allow the analysis means to produce data based on the analysis of the concentrated filtrate; data processing means for processing the data based on the analysis of the concentrated filtrate into information indicative of the effect of the sample on the enzyme reaction; and control means operatively connected to said dissolving and said dilution adding means, said sample solution pipetting means, said enzyme adding means, said substrate adding means, said moving means and said filtrate injecting means for automatically controlling the adding of the solvent by said dissolving solvent adding means and by said dilution solvent adding means, the injection of the diluted sample solution by said diluted sample solution pipetting means, the adding of the enzyme by said enzyme adding means, the adding of the substrate by said substrate adding means, the movement of said moving means, and the injection of the concentrated filtrate by said filtrate injecting means.