초록 ▼

Novel hybrid cell lines are disclosed for providing monoclonal antibodies to circulating parasite antigens present in the blood of animals infected with the nematode parasite Dirofilaria immitis. Such circulating parasite antigens are also characterized and identified. Further disclosed are methods

Novel hybrid cell lines are disclosed for providing monoclonal antibodies to circulating parasite antigens present in the blood of animals infected with the nematode parasite Dirofilaria immitis. Such circulating parasite antigens are also characterized and identified. Further disclosed are methods employed to produce and select such hybrid cell lines and diagnostically useful assay methods utilizing the monoclonal antibodies in the detection of such specific circulating parasite antigens in blood or bodily fluids.

대표청구항 ▼

An assay method for determining the presence of circulating parasite antigens of Dirofilaria immitis in the serum of plasma of D. immitis-infected dogs which comprises combining a sample of serum or plasma from a dog infected with Dirofilaria immitis and to which no parasite antigen or extract deriv

An assay method for determining the presence of circulating parasite antigens of Dirofilaria immitis in the serum of plasma of D. immitis-infected dogs which comprises combining a sample of serum or plasma from a dog infected with Dirofilaria immitis and to which no parasite antigen or extract derived from D. immitis is added with a first antibody bound to the surface of a solid support, adding thereto a conjugate composed of a second antibody and an enzyme and thereafter adding an enzyme substrate to indicate the presence of absence of said circulating parasite antigens in said sample.