초록 ▼

A process for culturing biological material capable of multiplication, especially cells securely adhering to microcarriers, employs a bioreactor having a vessel for a culture medium having means for controlling the environmental conditions in the culture medium and a stirring device for the homogene

A process for culturing biological material capable of multiplication, especially cells securely adhering to microcarriers, employs a bioreactor having a vessel for a culture medium having means for controlling the environmental conditions in the culture medium and a stirring device for the homogeneous distribution of the cells in the culture medium which has a rotary drive and a rotation axle running in the interior of the vessel, wherein the stirring device has at least one flat stirrer blade fixed on to the rotation axle and inclined to the rotation axle.

대표청구항 ▼

A process for culturing biological material capable of multiplication, which comprises the steps of: homogeneously suspending the biological material in a culture medium; placing said culture medium in a bioreactor comprising a vessel having a top wall and a bottom wall, means for controlling enviro

A process for culturing biological material capable of multiplication, which comprises the steps of: homogeneously suspending the biological material in a culture medium; placing said culture medium in a bioreactor comprising a vessel having a top wall and a bottom wall, means for controlling environmental conditions in the culture medium, and stirring means for the homogeneous distribution of the biological material in the culture medium, the stirring means having a rotation axle defining an axial direction and attached to a rotary drive means for driving the stirring means, said stirring means extending into and rotating in the interior of the vessel and comprising a plurality of stirrer blades in the form of plates affixed at adjacent points along the rotation axle with an angle of inclination toward said rotation axle, each stirrer blade having at its edge two extreme points displaced in the axial direction from the point at which the plate is fixed to the rotation axle, these extreme points having a predetermined distance in axial direction from each other, and wherein said points at which said stirrer blades are affixed to said rotation axle are separated from each other by a distance in the range of at least about half as great as said predetermined distance between said extreme points; and causing said stirrer blades to rotate, thereby keeping said biological material in homogeneous suspension while the biological material is multiplying.