초록 ▼

Antibody coated gold sol particles and antibody coated solid phase particles dispersed in an aqueous system react immunologically as a function of the presence of an analyte in a sample to be analyzed to produce a collectible, solid phase, gold-containing composite. The composite is collected on a f

Antibody coated gold sol particles and antibody coated solid phase particles dispersed in an aqueous system react immunologically as a function of the presence of an analyte in a sample to be analyzed to produce a collectible, solid phase, gold-containing composite. The composite is collected on a filter or at the bottom of a test tube by centrifugation or gravitation and the analyte in the sample is determined or detected by direct visual examination of the collected solid phase composite which has a pink or red or purplish coloration as a result of the gold contained thereby. The materials required for conducting the assay comprise coated gold particles, coated solid phase particles and a collector element such as a filter. The collected, solid phase, metal-containing composite, which may be directly visually examined to determine or detect the gold therein and thus the analyte in the sample, is stable and remains available for confirmation of test results at a later time.

대표청구항 ▼

In a process for the determination and detection of an immunologically reactive analyte in an aqueous sample, the steps of: (a) providing a labelled component comprising the coupling product of a first immunologically reactive substance and a metal-containing particle of a size and character to faci

In a process for the determination and detection of an immunologically reactive analyte in an aqueous sample, the steps of: (a) providing a labelled component comprising the coupling product of a first immunologically reactive substance and a metal-containing particle of a size and character to facilitate the maintenance of a stable, monodispersed suspension of the labelled component; (b) providing a solid phase component comprising the coupling product of a second immunologically reactive substance and a solid phase particle of a size and character to facilitate the maintenance of a stable suspension of the solid phase component and subsequent collection of a composite formed therefrom; (c) forming a mixed aqueous suspension of said components and which contains a sample to be analyzed for the analyte, said first and second substances being different and capable of binding directly or indirectly as a function of the presence of said analyte to thereby form a collectible, solid phase, metal-containing composite; (d) allowing the substances to bind to form said composite; (e) collecting the composite; and (f) determining or detecting the analyte in the sample by evaluating, through direct visual examination, the presence of metal in the collected solid phase composite.