초록 ▼

What are disclosed are a method for preventing the denaturation of proteins such as insulin in aqueous solution at interfaces by the addition to the solution of a surface-active substance comprising a chain of alternating weakly hydrophobic and weakly hydrophilic zones, protein solutions containing

What are disclosed are a method for preventing the denaturation of proteins such as insulin in aqueous solution at interfaces by the addition to the solution of a surface-active substance comprising a chain of alternating weakly hydrophobic and weakly hydrophilic zones, protein solutions containing such a surface-active substance, methods of purifying proteins contained in such solutions, and methods of treating surfaces with such a surface-active material to prevent the denaturation of proteins thereon.

대표청구항 ▼

A sterile aqueous solution of a water soluble biologically active protein, which solution has a pH between 6.8 and 8 and additionally has admixed therewith from 2 to 500 ppm of a surface active compound of the formula R2Y－Xn－R3 whereby said solution is stabilized to motion conditions, wherein Xn is

A sterile aqueous solution of a water soluble biologically active protein, which solution has a pH between 6.8 and 8 and additionally has admixed therewith from 2 to 500 ppm of a surface active compound of the formula R2Y－Xn－R3 whereby said solution is stabilized to motion conditions, wherein Xn is a chain of n members selected from the group of members having the formula [Figure] in any sequence, n is 2 to 80, Y is －O－or －NH－, R1 is －H, －CH3, or －C2H5, and may be the same or different but is such that at least one-half of the members of the chain Xn contains －CH3 or －C2H5, and R2 and R3 are each members independently selected from the group consisting of hydrogen, alkyl having 1 to 20 carbon atoms, carboxyalkyl having 2 to 20 carbon atoms, and alkylphenyl having 1 to 10 alkyl carbon atoms, but wherein R2 may only be such alkyl if Y is －NH－.