초록 ▼

An apparatus for the performance of efficient, large scale separations, particularly electrophoresis and isoelectric focusing, in free solution is described. Inner and outer cylinders define an annular separation chamber for solutions stabilized against convective flow by Taylor vortices generated d

An apparatus for the performance of efficient, large scale separations, particularly electrophoresis and isoelectric focusing, in free solution is described. Inner and outer cylinders define an annular separation chamber for solutions stabilized against convective flow by Taylor vortices generated during rotation of inner cylinder above threshold frequencies. Separation substrates in solution migrate independently in a potential field established between axially distal anodic and cathodic regions of the annular chamber. Axially defined portions of fluid in the chamber identified to contain concentrations of selected substrates are readily separated from other portions of fluid in the chamber.

대표청구항 ▼

A separation apparatus comprising a substantially uninterrupted annular chamber defined by walls of an outer cylinder and a substantially coaxial rotatably mounted inner cylinder, said annular chamber adapted to contain a support fluid and separation substrates capable of migrating relative to said

A separation apparatus comprising a substantially uninterrupted annular chamber defined by walls of an outer cylinder and a substantially coaxial rotatably mounted inner cylinder, said annular chamber adapted to contain a support fluid and separation substrates capable of migrating relative to said fluid under the influence of an electrical field, means for applying an electrical field to said chamber to generate an anodic region and an axially remote cathodic region in the annular chamber, means for rotating the inner cylinder relative to the outer cylinder at a rotational frequency (F) where F is at least as high the critical frequency (Fc) for onset of Taylor vortex flow in the annular chamber, and means for separating fluid in axially discrete portions of the annular chamber from fluid in other axially discrete portions of the annular chamber.