[특허]Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material

Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12Q-001/10 C12Q-001/08 C12Q-001/06 C12Q-001/04
출원번호	US-0880305 (1986-06-30)
발명자 / 주소	Edberg Stephen C. (356 Woodland La. Orange CT 06477)
인용정보	피인용 횟수 : 48 인용 특허 : 7

초록 ▼

The presence or absence of a predetermined target microbe in a sample is determined by adding a testing medium to the sample, or vice versa. The testing medium provides a selective growth medium for the target microbe and includes a specific nutrient which only the target microbe can metabolize. This specific nutrient is modified by attaching a sample-altering moiety thereto, thereby converting the nutrient to a nutrient-indicator. The sample-altering moiety is activated to alter the sample only if the specific nutrient is metabolized by the target microbe. The sample-altering moiety can be a material which changes the color of the sample (visible or non-visible) or changes an electrical characteristic of the sample, or alters some other detectable characteristic of the sample. The testing media does not have to be kept sterile, and the testing procedure does not have to be performed in a sterile environment.

대표청구항 ▼

A specific medium for combination with a specimen sample of a material suspected to be contaminated to determine the presence of absence of a target microbe in the specimen sample, and which can detect the presence of said target microbe without the need of performing a preliminary target microbe growth step, said medium comprising operative amounts of essential vitamins and elements needed to support growth of said target microbe and a nutrient-indicator which is the primary nutrient in the medium and which is substantially the only nutrient in said medium which can be metabolized by said target microbe to the extent needed to support continued reproductive growth thereof, and which cannot be metabolized by other viable microbes in the specimen, to that extent, said nutrient-indicator including a metabolizable moiety and a sample-altering moiety, the latter of which is released only when said nutrient-indicator is metabolized by said target microbe, whereupon a sensible characteristic of the sample is altered.

이 특허에 인용된 특허 (7)

Smith Robert E. (Livermore CA) Fox Eugene N. (Kensington CA), Composition, article and process for detecting a microorganism.
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D\Amato Richard F. (Dix Hills NY) Eriquez Louis A. (Ronkonkoma NY), Method, reagents and apparatus for the rapid identification of Neisseria gonorrhoeae and Neisseria meni.
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Koumura Ichiro (Sagamihara JPX) Kanou Hideo (Yokosuka JPX) Okunishi Masahiko (Tokyo JPX) Yamada Kazuhiko (Fujisawa JPX), Rapid method for detecting microorganisms.
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Bitton Gabriel (Gainesville FL) Koopman Ben (Gainesville FL), Assay pad and method for determination of the presence of total coliforms.
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Isbister Jenefir D., Biological assay for microbial contamination.
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Townsend, David E, Compositions and methods for detecting target microorganisms in a sample.
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Sharpin, Rosemary Katherine Cameron, Container for testing for micro-organisms.
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Edberg, Stephen C., Detection and quantification of one or more target analytes in a sample using spatially localized analyte reproduction.
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Edberg Stephen C., Detection of first generational environmental sourced microbes in an environmentally-derived sample.
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Pierson Mark W. ; Townsend David, Device and methods for determination of analyte in a solution.
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Chen Chun-Ming (Falmouth ME) Gu Haoyi (Portland ME), Medium for detecting Enterococci in a sample.
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Brenner Kristen P. ; Rankin Clifford C. ; Roybal Yvette R. ; Dufour Alfred P., Membrane filter agar medium containing two enzyme substrates used for the simultaneous detection of total coliforms and E. coli..
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David E. Townsend ; Chun-ming Chen, Method and composition for detecting bacterial contamination in food products.
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David E. Townsend ; Chun-ming Chen, Method and composition for detecting bacterial contamination in food products.
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Chen,Chung Ming; Edberg,Stephen C., Method and medium for detecting vancomycin-resistant.
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Grant Michael A., Method and medium for use in detecting E. coli and total coliforms.
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Jadamec Joseph Richard ; Bauman Robert ; Anderson Carol Patricia ; Jakubielski Stephen A. ; Sutton Neslie D. ; Kovacs Michael J., Method for detecting bacteria in a sample.
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Croteau Andrew J. (Old Orchard Beach ME) Pierson Mark W. (Saco ME) Townsend David E. (Scarborough ME) Naqui Ali (Falmouth ME), Method for quantification of biological material in a sample.
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Croteau Andrew J. ; Pierson Mark W. ; Townsend David E. ; Naqui Ali, Method for quantification of biological material in a sample.
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Pyle Barry H. ; McFeters Gordon A., Simple, rapid method for the detection, identification and enumeration of specific viable microorganisms.
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Gerritse, Jan; van Groenestijn, Johannes Wouterus; Zegers, Neeltje Dina, System for detecting microbial contamination.
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Dunkelberg, Hartmut, Test unit and method for testing whether a sterilizing packaging unit is effective against recontamination, and container packaging suitable for applying said method.
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Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material 원문보기

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Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material 원문보기

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