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Process for purifying DNA and RNA using cationic detergents 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C07H-021/00
  • C07H-021/02
  • C07H-021/04
출원번호 US-0376709 (1989-07-07)
발명자 / 주소
  • Macfarlane Donald E. (943 Iowa Ave. Iowa City IA 52240)
인용정보 피인용 횟수 : 66  인용 특허 : 3

초록

A novel method is provided for purifying DNA and RNA from a variety of sources, including cells, cell lysates, viruses, tissues, blood and other body fluids employing a cationic detergent to complex with the nucleic acids.

대표청구항

A process for the purification of DNA and RNA from a biological mixture consisting essentially of applying directly to said mixture without prior purification or extraction of the nucleic acids a selected quaternary amine cationic detergent capable of simultaneously solubilizing contaminants in said

이 특허에 인용된 특허 (3)

  1. Hodge Richard Anthony Vere (Dorking EN), Antiviral ionic complexes of double-standard RNA.
  2. DeBonville David A. (Cambridge MA) Riedel Gerard E. (Concord MA), Method for the isolation and purification of DNA molecules.
  3. Chomczynski Piotr (727 Martin Luther King Dr. Cincinnati OH 45220), Product and process for isolating RNA.

이 특허를 인용한 특허 (66)

  1. Cronce Donald T., Chemical warfare agent decontaminant solution using quaternary ammonium complexes.
  2. Bair, Robert Jackson; Benedict, Kristen Campbell; Kivens, Wendy J.; Kwiatkowski, Jr., Robert W.; Paulsen, Kim; Strom, Daniel A.; Wages, John M., Compositions and methods for using a solid support to purify DNA.
  3. Bair, Jr., Robert Jackson; Heath, Ellen M.; Meehan, Heather; Paulsen, Kim Elayne; Wages, Jr., John M., Compositions and methods for using a solid support to purify RNA.
  4. Bair, Jr.,Robert Jackson; Heath,Ellen M.; Meehan,Heather; Paulsen,Kim Elayne; Wages, Jr.,John M., Compositions and methods for using a solid support to purify RNA.
  5. Holl채nder,Vera; Wyrich,Ralf; Oelm체ller,Uwe, Compositions for isolating and/or stabilising nucleic acids in biological material.
  6. Holländer, Vera; Wyrich, Ralf; Oelmüller, Uwe, Compositions for the isolation and/or stabilization of nucleic acids in biological materials.
  7. Greenfield, Lawrence; Montesclaros, Luz, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
  8. Greenfield,I. Lawrence, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
  9. Greenfield,Lawrence; Montesclaros,Luz, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
  10. Pasloske, Brittan L.; Fang, Xingwang; Hoang, Quoc, Crude biological derivatives competent for nucleic acid detection.
  11. Lienau, E. Kurt; Hurley, J. Michael, DNA isolation method and kit.
  12. Gray Thomas Kevin ; Doll Mark A., DNA purification procedure.
  13. Cronce Donald T., Decontamination solution and method.
  14. Rill Randolph L. ; Liu Zhi-Ren, Dicationic perylene dye for precipitation and quantitation of DNA.
  15. Schneider Claudio (Udine ITX), Extraction of genomic DNA from blood using cationic detergents.
  16. Paulsen,Kim E., Formulations and methods for denaturing proteins.
  17. Heath Ellen M., Low pH RNA isolation reagents, method, and kit.
  18. Haddad, Louis C.; Swenson, Barbara C.; Bothof, Catherine A.; Raghavachari, Madhusudan, Materials, methods, and kits for reducing nonspecific binding of molecules to a surface.
  19. Peters, Lars-Erik, Method and compositions for reversible inhibition of thermostable polymerases.
  20. Augello, Frank A.; Rainen, Lynne; Walenciak, Matthew; Oelmuller, Uwe; Wyrich, Ralf; Bastian, Helge, Method and device for collecting and stabilizing a biological sample.
  21. Bauer, Stefan, Method for detecting a periodically pulsing operating parameter.
  22. Hayashizaki, Yoshihide; Carninci, Piero, Method for isolating DNA.
  23. Yoshihide Hayashizaki JP; Piero Carninci JP, Method for isolating DNA.
  24. Voss, Thorsten, Method for isolating nucleic acids.
  25. Little Michael C. ; Llorin Oscar J. ; Collis Matthew P., Method for reducing inhibitors of nucleic acid hybridization.
  26. Korfhage, Christian; Wilmer, Friederike; Loffert, Dirk; Himmelreich, Ralf; Fritz, Claudia; Rieske, Kathleen, Method for the treatment of a sample containing biomolecules.
  27. Korfhage, Christian; Wilmer, Friederike; Löffert, Dirk; Himmelreich, Ralf; Fritz, Claudia; Rieske, Kathleen, Method for the treatment of a sample containing biomolecules.
  28. Korfhage, Christian; Wilmer, Friederike; Löffert, Dirk; Himmelreich, Ralf; Fritz, Claudia; Rieske, Kathleen, Method for the treatment of a sample containing biomolecules.
  29. Okada, Yoshikatsu, Method of producing probe medium and method of immobilizing probe using probe medium.
  30. Erbacher, Christoph; Bastian, Helge; Wyrich, Ralf; Oelmüller, Uwe; Manz, Thomas, Method of stabilizing and/or isolating nucleic acids.
  31. Heath, Ellen M.; Morken, Nathaniel W.; Benedict, Kristen Campbell, Method to isolate DNA.
  32. Han,Xiaoliang; Liu,Zhongdong; Xia,Jinming, Methods and compositions to extract DNA, RNA and protein simultaneously from biological samples.
  33. Rosario-Jansen, Theresa; Wright, David Erick, Methods and kits for predicting infusion reaction risk and antibody-mediated loss of response by monitoring serum uric acid during PEGylated uricase therapy.
  34. Lader Eric S., Methods and reagents for preserving RNA in cell and tissue samples.
  35. Hartman, Jacob; Mendelovitz, Simona; Rehrig, Claudia D.; Huang, William; Hershfield, Michael, Methods for lowering elevated uric acid levels using intravenous injections of PEG-uricase.
  36. O'Hagan, Derek; Singh, Manmohan; Ott, Gary, Microparticles with adsorbent surfaces, methods of making same, and uses thereof.
  37. Gundling, Gerard, Nucleic acid isolation method and kit.
  38. Williams, L. David; Hershfield, Michael S.; Kelly, Susan J.; Saifer, Mark G. P.; Sherman, Merry R., PEG-urate oxidase conjugates and use thereof.
  39. Bloch Will, Precision and accuracy of anion-exchange separation of nucleic acids.
  40. Collis, Matthew P.; Copertino, Donald W.; Eckert, Karen; Fort, Thomas L., Pretreatment method for extraction of nucleic acid from biological samples and kits therefor.
  41. Collis, Matthew P.; Copertino, Donald W.; Eckert, Karen; Fort, Thomas L., Pretreatment method for extraction of nucleic acid from biological samples and kits therefor.
  42. Chomczynski Piotr, Product and process for isolating DNA, RNA and proteins.
  43. Fischer, Meir; Harosh, Eliyahu, Purification of proteins with cationic surfactant.
  44. Macfarlane Donald E., Quaternary amine surfactant and methods of using same in isolation of RNA.
  45. Macfarlane Donald E., Quaternary amine surfactants and methods of using same in isolation of RNA.
  46. Belly,Robert; Toner,Jacqueline; Backus,John, Rapid extraction of RNA from cells and tissues.
  47. Baker, Tony, Removal of molecular assay interferences.
  48. Goldsborough, Andres Simon; Bates, Malcolm Robert, Sample fixation and stabilisation.
  49. Nanibhushan Dattagupta ; C. Nagaraja Sridhar ; Whei-Kuo Wu, Sample processing to release nucleic acids for direct detection.
  50. Randolph, Theodore W.; Manning, Mark C.; Falk, Richard F., Sustained-release composition including amorphous polymer.
  51. Oelm?ller, Uwe; Wille, Tanja, Use of compositions consisting of cationic compounds and proton donors for stabilizing and/or isolating nucleic acids in or from micro-organisms such as prokaryots, fungi, protozoa or algae.
  52. O'Hagan, Derek; Van Nest, Gary; Ott, Gary S.; Barackman, John; Kazzaz, Jina, Use of microparticles with adsorbed antigen to stimulate immune responses.
  53. Bedingham, William; Robole, Barry W.; Parthasarathy, Ranjani V.; Ericson, Katya, Variable valve apparatus and methods.
  54. Hartman, Jacob; Mendelovitz, Simona, Variant form of urate oxidase and use thereof.
  55. Hartman, Jacob; Mendelovitz, Simona, Variant form of urate oxidase and use thereof.
  56. Hartman, Jacob; Mendelovitz, Simona, Variant form of urate oxidase and use thereof.
  57. Hartman, Jacob; Mendelovitz, Simona, Variant form of urate oxidase and use thereof.
  58. Hartman, Jacob; Mendelovitz, Simona, Variant form of urate oxidase and use thereof.
  59. Hartman, Jacob; Mendelovitz, Simona, Variant forms of urate oxidase and use thereof.
  60. Hartman, Jacob; Mendelovitz, Simona, Variant forms of urate oxidase and use thereof.
  61. Hartman, Jacob; Mendelovitz, Simona, Variant forms of urate oxidase and use thereof.
  62. Hartman, Jacob; Mendelovitz, Simona, Variant forms of urate oxidase and use thereof.
  63. Hartman, Jacob; Mendelovitz, Simona, Variant forms of urate oxidase and use thereof.
  64. Hartman, Jacob; Mendelovitz, Simona, Variant forms of urate oxidase and use thereof.
  65. Hartman, Jacob; Mendelovitz, Simona, Variant forms of urate oxidase and use thereof.
  66. Helftenbein, Elke, Vessel for blood sampling.
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