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Process for chemical manipulation of non-aqueous surrounded microdroplets 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/02
  • C12Q-001/18
  • C12N-011/04
출원번호 US-0185156 (1988-04-22)
발명자 / 주소
  • Weaver James C. (Sudbury MA) Williams Gregory B. (Tewksbury MA) Bliss Jonathan G. (Somerville MA)
출원인 / 주소
  • Massachusetts Institute of Technology (Cambridge MA 02)
인용정보 피인용 횟수 : 43  인용 특허 : 0

초록

A process for the chemical manipulation of liquid and gel microdroplets is disclosed. The process involves providing first microdroplets having aqueous interiors, such that the first microdroplets are surrounded by a non-aqueous fluid. The aqueous interior chemical composition of the first microdrop

대표청구항

A method for detecting an interaction between a water-soluble compound and at least one biological entity, comprising the steps of: a) disposing a biological entity in an aqueous medium, wherein the biological entity is selected from the group consisting of microorganisms, cells, protoplasts, small

이 특허를 인용한 특허 (43)

  1. Clague,David S.; Wheeler,Elizabeth K.; Lee,Abraham P., Amphiphilic mediated sample preparation for micro-flow cytometry.
  2. Clague,David S.; Wheeler,Elizabeth K.; Lee,Abraham P., Amphiphilic mediated sample preparation for micro-flow cytometry.
  3. Hindson, Benjamin J.; Colston, Jr., Billy W.; Ness, Kevin D.; Masquelier, Donald A., Calibrations and controls for droplet-based assays.
  4. Ness, Kevin Dean; Burd, Samuel; Hindson, Benjamin Joseph; Belgrader, Phillip; Colston, Jr., Billy W., Cartridge with lysis chamber and droplet generator.
  5. Ness, Kevin D.; Arbore, Mark A.; Hurst, Jerry E.; Klein, David L.; Masquelier, Donald A., Detection system for droplet-based assays.
  6. Ness, Kevin D.; Hindson, Benjamin J.; Colston, Jr., Billy W.; Masquelier, Donald A., Device for generating droplets.
  7. Ness, Kevin D.; Kelly, Christopher F.; Masquelier, Donald A., Device for generating droplets.
  8. Luo, Guobin; Sedova, Marina; Light, David; Jones, Ryan; Alanjary, Mohammad, Device preparation using condensed nucleic acid particles.
  9. Saxonov, Serge; Dube, Simant; Regan, John Frederick, Digital assays with multiplexed detection of two or more targets in the same optical channel.
  10. Ness, Kevin D.; Kelly, Christopher F.; Masquelier, Donald A., Droplet generation for droplet-based assays.
  11. Ness, Kevin D.; Hindson, Benjamin J.; Makarewicz, Jr., Anthony J.; Hiddessen, Amy L., Droplet transport system for detection.
  12. Colston, Jr., Billy Wayne; Hindson, Benjamin Joseph; Ness, Kevin Dean; Masquelier, Donald Arthur; Milanovich, Fred Paul; Modlin, Douglas N.; Riot, Vincent; Burd, Samuel; Makarewicz, Jr., Anthony Joseph; Belgrader, Phillip, Droplet-based assay system.
  13. Hiddessen, Amy L.; Hindson, Benjamin J., Emulsion chemistry for encapsulated droplets.
  14. Ness, Kevin D.; Masquelier, Donald A.; Colston, Jr., Billy W.; Hindson, Benjamin J., Flow-based thermocycling system with thermoelectric cooler.
  15. Short, Jay M.; Keller, Martin, High throughput screening for novel Bioactivities.
  16. Short Jay M. ; Keller Martin, High throughput screening for novel enzymes.
  17. Short, Jay M.; Keller, Martin, High throughput screening of mycelia for bioactivities or biomolecules.
  18. Saxonov, Serge; Tzonev, Svilen S.; Lucero, Michael Y.; Koehler, Ryan T.; Hindson, Benjamin J., Library characterization by digital assay.
  19. Short, Jay M.; Keller, Martin, Method for high throughput screening of an environmental library.
  20. Hindson, Benjamin J.; Ness, Kevin D.; Colston, Jr., Billy W.; Milanovich, Fred P.; Masquelier, Donald A., Method of generating droplets.
  21. Walker Harrell L. ; Patrick Christopher L., Method of isolating and propagating microorganisms and viruses.
  22. Ness, Kevin D.; Hindson, Benjamin J.; Colston, Jr., Billy W.; Masquelier, Donald A., Method of making a device for generating droplets.
  23. Hindson, Benjamin J.; Colston, Jr., Billy W.; Ness, Kevin D.; Masquelier, Donald A., Method of mixing fluids by coalescence of multiple emulsions.
  24. Saxonov, Serge, Methods and compositions for nucleic acid analysis.
  25. Saxonov, Serge, Methods and compositions for nucleic acid analysis.
  26. Saxonov, Serge, Methods and compositions for nucleic acid analysis.
  27. Saxonov, Serge; Dube, Simant; Hindson, Benjamin J.; McCoy, Adam M., Multiplexed digital assays with combinatorial use of signals.
  28. Light, David; Rosenblum, Barnett, Polymer particles, nucleic acid polymer particles and methods of making and using the same.
  29. Light, David; Rosenblum, Barnett B., Polymer particles, nucleic acid polymer particles and methods of making and using the same.
  30. Light, David; Rosenblum, Barnett B., Polymer particles, nucleic acid polymer particles and methods of making and using the same.
  31. Cauley, III, Thomas H.; Bousse, Luc; Petersen, Jonathan; Ness, Kevin D.; Romine, Steven, Sample holder with a well having a wicking promoter.
  32. Makarewicz, Anthony J.; Hiddessen, Amy L., System for detection of spaced droplets.
  33. Makarewicz, Jr., Anthony J.; Hiddessen, Amy L., System for detection of spaced droplets.
  34. Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for droplet-based assays using an array of emulsions.
  35. Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for forming an array of emulsions.
  36. Ness, Kevin D.; Hindson, Benjamin J.; Colston, Jr., Billy W.; Masquelier, Donald A., System for forming an array of emulsions.
  37. Hiddessen, Amy L.; Masquelier, Donald A.; Ness, Kevin D.; Hindson, Benjamin J.; Makarewicz, Jr., Anthony J.; Chia, Erin R., System for forming emulsions.
  38. Hiddessen, Amy L.; Ness, Kevin D.; Hindson, Benjamin J.; Masquelier, Donald A.; Chia, Erin R., System for generating droplets with pressure monitoring.
  39. Hiddessen, Amy L.; Ness, Kevin D.; Hindson, Benjamin J.; Masquelier, Donald A.; Chia, Erin R., System for generating droplets with push-back to remove oil.
  40. Hiddessen, Amy L.; Ness, Kevin D.; Hindson, Benjamin J.; Masquelier, Donald A., System for generating droplets—instruments and cassette.
  41. Hindson, Benjamin J., System for hot-start amplification via a multiple emulsion.
  42. Hindson, Benjamin J.; Colston, Jr., Billy W.; Ness, Kevin D.; Masquelier, Donald A., System for mixing fluids by coalescence of multiple emulsions.
  43. Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for transporting emulsions from an array to a detector.
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