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DNA sequencing using fluorescence background electroblotting membrane 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/68
출원번호 US-0365693 (1989-06-14)
발명자 / 주소
  • Caldwell Karin D. (Salt Lake City UT) Chu Tun-Jen (Salt Lake City UT) Pitt William G. (Orem UT)
출원인 / 주소
  • University of Utah (Salt Lake City UT 02) Brigham Young University (Provo UT 02)
인용정보 피인용 횟수 : 92  인용 특허 : 0

초록

A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence w

대표청구항

A method for the multiplex sequencing of DNA comprising: (a) providing a target DNA sample to be sequenced wherein the DNA fragments have been chain terminated and separated according to a specific adenine, guanine, cytosine or thymine base groupings; (b) subjecting base separated groupings of targe

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