초록 ▼

A method and composition for the enzymatic assay of liquid test sample, such as urine or serum, for a specific analyte, such as glucose. The method utilizes a dry phase test strip comprising a carrier matrix impregnated with a stabilized enzyme reagent composition including an oxidase enzyme, such a

A method and composition for the enzymatic assay of liquid test sample, such as urine or serum, for a specific analyte, such as glucose. The method utilizes a dry phase test strip comprising a carrier matrix impregnated with a stabilized enzyme reagent composition including an oxidase enzyme, such as glucose oxidase, that is capable of interacting with the analyte of interest; a chromogen that changes color in response to the analyte-oxidase enzyme interaction to reveal the presence and/or concentration of the analyte in the test sample; and a stabilizer, such as a hydrazide, like GIRARD\S Reagent T, or an amino acid, like arginine, or an amino acid derivative, like the tris(hydroxymethyl)aminomethane salt of glutamic acid, to improve the heat stability and shelf life of the dry phase test strip.

대표청구항 ▼

An enzyme reagent composition for incorporation into a dry phase test strip capable of exhibiting a sufficient color transition upon contacting an analyte determined from the group consisting of glucose, cholesterol, uric acid and lower alcohols to show the presence or concentration of the analyte b

An enzyme reagent composition for incorporation into a dry phase test strip capable of exhibiting a sufficient color transition upon contacting an analyte determined from the group consisting of glucose, cholesterol, uric acid and lower alcohols to show the presence or concentration of the analyte being determined in a liquid test sample and capable of maintaining enzymatic activity over an extended time period comprising: an oxidase enzyme for the analyte being determined; an oxidizable chromogen; peroxidase; a buffer capable of buffering the enzyme reagent composition to a pH in the range of from about 4 to about 7; and a hydrazine selected from the group consisting of hydrazine monohydrate, (carboxymethyl)trimethylammonium chloride hydrazide, 1-(carboxymethyl)-pyridinium chloride hydrazide, 2-hydrazine-2-imidazoline hydrobromide and 2-furoic acid hydrazide.