초록

There is provided a method for producing an active ingredient isolated from the cortex of Mimosa tenuiflora by solvent extraction. The active ingredient has epidermal regenerative properties such as utility in the treatment of burns.

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A process for producing a Mimosa tenuiflora cortex extract which exhibits the following properties: a) odorless, reddish coffee-colored powder having a bitter taste; b) soluble in ethanol, DMSO, methanol and acetone; c) insoluble in water, chloroform, ethyl acetate, ethyl ether, propanol, hexane, CC

A process for producing a Mimosa tenuiflora cortex extract which exhibits the following properties: a) odorless, reddish coffee-colored powder having a bitter taste; b) soluble in ethanol, DMSO, methanol and acetone; c) insoluble in water, chloroform, ethyl acetate, ethyl ether, propanol, hexane, CCl4, THF, dioxane, amyl alcohol, isopropyl alcohol, benzyl alcohol, and diethylamine; d) a melting point of about 222°C.; e) has the following Rf values in thin layer chromatography with the following eluting systems: ________________________________ - Eluting System Rf ________________________________ Chloroform 0 ethyl acetate 0 petroleum ether 0 benzene 0 methanol-water-DMSO (1:1:1) v/v 0.963 ethanol-water-acetic acid (2:1:1) v/v 0.817 methanol-DMSO 0.910 DMSO 1.000 dioxane-methanol-ethyl acetate (1:1:1) v/v 0 ________________________________ f) contains no heavy elements; g) exhibits an ultraviolet absorption spectrum in ethanol solution, with absorption and absorbance maxima of 222 nm (a=2.840) and 281 nm (a=0.600); h) exhibits an IR absorption spectrum (in potassium bromide tablet) with main absorption bands at: 3290, 2910, 1630, 1530, 1450, 1190, 1150, 1110, 1020, 840, 620, 580 cm-1; i) exhibits a proton NMR spectrum in D2O with signs at: 1.13 (m), 3.36 (m), 4.33 (s), 5.1 (s), 6.24 (d), 8.01 (s), 8.81 (s), 9.01 (s), 9.98 (s), ppm; j) exhibits a 13C NMR spectrum in D2O with signs at: 17.5, 18.25, 18.75, 19.5, 20.02, 21, 23, 25, 25.5, 26.25, 27.5, 27.9, 32, 32.8, 33.56, 36.4, 36.52, 37.5, 42, 42.2, 43, 45.2, 47.2, 49.2, 50.2, 52.54, 55, 59.5, 60, 60.2, 62.7, 74.7, 65, 67.2, 68, 68.1, 69, 69.9, 70.5, 71.7, 74.2, 75.76, 76.5, 77, 78.2, 81.2, 82.5, 83, 89, 96, 98, 98.7, 100, 102, 103.2, 103.5, 105, 105.2, 105.5, 106.5, 107.5, 109.7, 112, 112.2, 112.5, 112.7, 115, 115.7, 117.5, 118.5, 119.7, 123.5, 127, 128, 128.8, 129, 130, 131.5, 132, 136.9, 137.5, 143, 144.2, 144.7, 144.5, 146.2, 149.5, 150.5, 151, 152.7, 153.5, 153.6, 153.7, 155.5, 156, 157, 158, 160, 164, 163, 166, 167 (only up to 170 was read); k) the elemental analysis is as follows: C: 54-57% H: 4.5-5.4% O: 36.5-37.5% which comprises subjecting ground Mimosa tenuiflora cortex to successive extractions with solvents by the steps of i) grinding the Mimosa tenuiflora cortex until a powder is obtained,