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| 국가/구분 | United States(US) Patent 등록 |
|---|---|
| 국제특허분류(IPC7판) |
|
| 출원번호 | US-0160595 (1988-02-26) |
| 발명자 / 주소 |
|
| 출원인 / 주소 |
|
| 인용정보 | 피인용 횟수 : 250 인용 특허 : 0 |
Oxygen-independent methods, systems and devices for the enzymatic colorimetric assay and detection of biochemical analytes. Two systems are described, both of which produce less than one equivalent of dye per equivalent of substrate, maintaining dye concentrations in the range where Beer\s law predi
Oxygen-independent methods, systems and devices for the enzymatic colorimetric assay and detection of biochemical analytes. Two systems are described, both of which produce less than one equivalent of dye per equivalent of substrate, maintaining dye concentrations in the range where Beer\s law predicts a linear color-concentration relationship. One system produces an analog color signal from an analog analyte input, the other system produces a digital color signal from an analog analyte input.
A colorimetric assay system which is designed to measure the presence or concentration of a biochemical analyte in a sample by a color change which is indicative of the presence or concentration of the analyte, wherein the system comprises, (a) an oxidase enzyme capable of oxidizing the analyte by r
A colorimetric assay system which is designed to measure the presence or concentration of a biochemical analyte in a sample by a color change which is indicative of the presence or concentration of the analyte, wherein the system comprises, (a) an oxidase enzyme capable of oxidizing the analyte by removing electrons from the analyte without involving oxygen in the reaction, thereby yielding a reduced enzyme, (b) a non-chromogenic electron acceptor which is oxygen, having a half cell reduction potential greater than that of the reduced enzyme, wherein the electron acceptor is capable of accepting electrons from the reduced enzyme in a functionally irreversible manner, and (c) a chromogen which is capable of accepting electrons from the reduced enzyme and changing color in a visible range upon reduction, wherein the change of color is less than one equivalent of color per equivalent of analyte oxidized and is indicative of the presence or the concentration of the analyte to be determined.
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