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특허 상세정보

MyON담기
내보내기 페이스북 공유 카카오톡 공유

Methods of extracting nucleic acids and PCR amplification without using a proteolytic enzyme

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12P-019/34   
미국특허분류(USC) 435/91 ; 435/6 ; 435/1723 ; 435/259
출원번호 US-0423071 (1989-10-18)
발명자 / 주소
출원인 / 주소
인용정보 피인용 횟수 : 55  인용 특허 : 0
초록
▼

This invention provides a rapid and highly effective method for extracting nucleic acids from cells or virions without the use of proteolytic enzymes. Extraction is accomplished within a few minutes using a lysing composition comprising a buffer, a source of a DNA polymerase cofactor, a stabilizer and at least one nonionic surfactant which will release nucleic acids from cytoplasmic and nuclear membranes of cells or virions. The resulting mixture is heated to boiling for up to fifteen minutes, and the nucleic acids are recovered for amplification using p...

대표
청구항
▼

A method for amplifying a targeted nucleic acid using polymerase chain reaction, said method comprising: I. extracting deoxyribonucleic acids from cells or virions in a sample of human or animal whole blood or a component thereof using an extraction procedure consisting essentially of: A. mixing said sample with a lysing composition comprising: (1) an organic buffer which maintains said composition at a pH of from about 4 to about 10, (2) a catalytic amount of a free metal ion cofactor for DNA polymerase activity, (3) a stabilizing material, (4) at leas...

이 특허를 인용한 특허 피인용횟수: 55

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  3. Boyden, Edward S.; Cook, Daniel B.; Hyde, Roderick A.; Leuthardt, Eric C.; Myhrvold, Nathan P.; Sweeney, Elizabeth A.; Wood, Jr., Lowell L.. Compositions and methods for administering compartmentalized frozen particles. USP2013108551506.
  4. Boyden, Edward S.; Cook, Daniel B.; Hyde, Roderick A.; Leuthardt, Eric C.; Myhrvold, Nathan P.; Sweeney, Elizabeth A.; Wood, Jr., Lowell L.. Compositions and methods for administering compartmentalized frozen particles. USP2013128603494.
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