초록 ▼

A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA binding protein can be used to screen for novel ligands. The screen

A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.

대표청구항 ▼

A method for screening a random peptide library to identify a plasmid that encodes a peptide that binds to a receptor, said library comprising at least 106 different transformed E. coli host cells, each cell containing a plasmid that comprises a lacO binding site and encodes a fusion protein compris

A method for screening a random peptide library to identify a plasmid that encodes a peptide that binds to a receptor, said library comprising at least 106 different transformed E. coli host cells, each cell containing a plasmid that comprises a lacO binding site and encodes a fusion protein comprising a lac repressor DNA binding protein fused to a peptide, wherein said 106 different transformed host cells each differ from one another with respect to the peptide in said fusion protein, said method comprising the steps of: (a) lysing the transformed host cells under conditions such that the fusion protein remains bound to the vector plasmid that encodes the fusion protein; (b) contacting the fusion proteins of the peptide library with a receptor under conditions conducive to specific peptide－receptor binding; and (c) isolating the plasmid that encodes a peptide that binds to said receptor.