초록

The present invention provides an improved process for preparing coated microbial pesticides comprising antagonistic microorganisms and natural biopolymer originated from natural sources; and pesticides prepared therefrom.

대표청구항 ▼

A process for preparing a microbial pesticide which consists essentially of (a) mixing with water and heating a biopolymer selected from the group consisting of microbiologically metabolizable biopolymer-containing natural substances and microbiologically metabolizable biopolymers extracted therefro

A process for preparing a microbial pesticide which consists essentially of (a) mixing with water and heating a biopolymer selected from the group consisting of microbiologically metabolizable biopolymer-containing natural substances and microbiologically metabolizable biopolymers extracted therefrom, into the form of a gel or paste at a temperature between 80°C. and 121°C., wherein said biopolymer is one or more substances selected from the group consisting of grains, tubers, tuberous roots and starch extracted therefrom; agar; gelatin, polygalacturonic acid; chitosan; carboxymethyl cellulose; gelrite; and natural wax; (b) cooling said biopolymer gel or paste to a temperature between a room temperature and 60°C.; (c) mixing one or more antagonistic microorganisms in an amount ranging from about 104 to 1014 cells per gram of the pesticide, in biologically pure cultures, selected from the group consisting of Bacillus subtilis subsp. Krictiensis ATCC 55078 and 55079, Pseudomonas pyrocinia ATCC 15958, Pseudomonas fluorescence ATCC 27663, Gliocladium virens ATCC 52045, Trichoderma reesei ATCC 28217, Trichoderma harzianum ATCC 52445, Trichoderma hamatum ATCC 52198, Trichoderma viride ATCC 52440, Streptomyces cacaoi subsp. asoensis ATCC 19093, Bacillus thuringiensis ATCC 13367, Beauveria bassiana ATCC 26851, 48585 and 48023, Hirsutella thomsonii ATCC 24874, Metarhizium flavoviride ATCC 32969, Verticillium lencanii ATCC 46578 and Collectotrichum gloeosporioides f. sp. jussiaeas ATCC 52634 with said cooled biopolymer gel or paste; and then (d) formulating and drying the microorganism-biopolymer gel or paste mixture; whereby an unfermented microbial pesticide is produced.