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Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/02
  • C12Q-001/68
  • C12Q-001/04
출원번호 US-0783182 (1991-10-28)
발명자 / 주소
  • Haugland Richard P. (Eugene OR) MacCoubrey Ian C. (Blacksburg VA) Moore Patrick L. (West Roxbury MA)
출원인 / 주소
  • Molecular Probes, Inc. (Eugene OR 02)
인용정보 피인용 횟수 : 62  인용 특허 : 0

초록

This invention relates to a method for simultaneously detecting live and dead cells using two fluorogenic reagents: calcein AM and ethidium homodimer. Live cells are distinguished by an intense uniform green fluorescence generated by the enzymatic hydrolysis of calcein AM: [Figure] Dead or damaged c

대표청구항

A method for evaluating the viability of cells in a sample, comprising: a) combining a sample containing cells with a concentration of calcein AM sufficient to yield detectable green fluorescence in any live cells present in said sample and a concentration of ethidium homodimer sufficient to yield d

이 특허를 인용한 특허 (62)

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