초록 ▼

Disclosed are (1) osteogenic devices comprising a matrix containing substantially pure natural-sourced mammalian osteogenic protein; (2) DNA and amino acid sequences for novel polypeptide chains useful as subunits of dimeric osteogenic proteins; (3) vectors carrying sequences encoding these novel po

Disclosed are (1) osteogenic devices comprising a matrix containing substantially pure natural-sourced mammalian osteogenic protein; (2) DNA and amino acid sequences for novel polypeptide chains useful as subunits of dimeric osteogenic proteins; (3) vectors carrying sequences encoding these novel polypeptide chains and host cells transfected with these vectors; (4) methods of producing these polypeptide chains using recombinant DNA technology; (5) antibodies specific for these novel polypeptide chains; (6) osteogenic devices comprising these recombinantly produced proteins in association with an appropriate carrier matrix; and (7) methods of using the osteogenic devices to mimic the natural course of endochondral bone formation in mammals.

대표청구항 ▼

In an osteogenic device for implantation in a mammal, the device comprising: osteogenic protein dispersed within a biocompatible, in vivo biodegradeable matrix defining pores of a dimension sufficient to permit influx, differentiation, and proliferation of migratory progenitor cells from the body of

In an osteogenic device for implantation in a mammal, the device comprising: osteogenic protein dispersed within a biocompatible, in vivo biodegradeable matrix defining pores of a dimension sufficient to permit influx, differentiation, and proliferation of migratory progenitor cells from the body of said mammal, the improvement wherein said osteogenic protein comprises a pair of unglycosylated protein chains disulfide bonded to produce a dimeric species having a conformation such that said pair of unglycosylated polypeptide chains is capable of inducing endochondral bone formation in said mammal when disposed in said matrix and accessible to said cells, said osteogenic protein being substantially pure, and comprising at least one of the following amino acid sequences: a) Ala-Cys-Cys-Val-Pro-Thr-Glu-Leu-Ser-Ala-Ile Ser-Met-Leu-Tyr-Leu-Asp-Glu-Asn-Glu-Lys, b) Val-Pro-Lys-Pro, and c) Ala-Pro-Thr.