초록

Systems and methods resuspend cellular-rich concentrates of blood by rocking the separation chamber to create variable agitation forces within the separation chamber. The systems and methods also add resuspension fluids to aid the resuspension.

대표청구항 ▼

A method for collecting a resuspended platelet-rich concentrate comprising the steps of (a) rotating a separation chamber about an axis to create centrifugal forces within the chamber, while: (i) continuously conveying a suspension of platelets into the separation chamber for separation in response

A method for collecting a resuspended platelet-rich concentrate comprising the steps of (a) rotating a separation chamber about an axis to create centrifugal forces within the chamber, while: (i) continuously conveying a suspension of platelets into the separation chamber for separation in response to the centrifugal forces into a platelet-rich concentrate and a platelet-poor component, (ii) continuously conveying the platelet-poor component from the separation chamber, leaving the platelet-rich concentrate behind in the separation chamber, and (iii) continuously collecting a predetermined portion of the platelet-poor component in a reservoir, (b) terminating rotation of the separation chamber and the conveyance of platelet suspension into the separation chamber when a desired yield of platelet-rich concentrate occupies the chamber, (c) removing about one-half of the fluid volume occupying the chamber by conveying platelet-poor component from the separation chamber to the reservoir, (d) rocking the separation chamber, with about one-half of its fluid volume removed, to create intensified variable agitation forces within the separation chamber, (e) returning platelet-poor component from the reservoir to the chamber, while rocking the separation chamber, in a volume sufficient to essentially replace the fluid volume removed in step (c), to thereby at least partially resuspend platelet-rich concentrate within the separation chamber in response to the agitation forces, (f) conveying a partial volume of platelet-rich concentrate, resuspended in returned platelet-poor component, from the separation chamber to a collection container, leaving a remaining volume of platelet-rich concentrate occupying the separation chamber, and repeating steps (c) to (f) in succession until substantially all of the platelet-rich concentrate is resuspended with returned platelet-poor component and is conveyed to the collection container.