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Overproduction and purification of soluble PHA synthase 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12N-009/88
  • C12N-015/60
출원번호 US-0290131 (1994-08-12)
발명자 / 주소
  • Peoples Oliver P. (Arlington MA) Gerngross Tillman U. (Cambridge MA) Sinskey Anthony J. (Boston MA)
출원인 / 주소
  • Massachusetts Institute of Technology and Metabolix, Inc. (Cambridge MA 02)
인용정보 피인용 횟수 : 75  인용 특허 : 0

초록

Purified, soluble recombinant bacterial long chain and short chain PHA synthases are described, with methods and materials for overexpression and purification.

대표청구항

A method for the overproduction and purification of soluble polyhydroxyalkanoate (PHA) synthase expressed from an isolated structural gene comprising expressing the isolated structural gene encoding the synthase in a vector in an expression system not expressing an enzyme cleaving the synthase selec

이 특허를 인용한 특허 (75)

  1. Martin, David P.; Skraly, Frank A., Bioabsorbable polymer containing 2-hydroxyacid monomers.
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  13. Williams, Simon F.; Martin, David P.; Skraly, Frank A., Medical devices and applications of polyhydroxyalkanoate polymers.
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  15. Williams, Simon F.; Martin, David P.; Skraly, Frank A., Medical devices and applications of polyhydroxyalkanoate polymers.
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  17. Williams,Simon F.; Martin,David P.; Skraly,Frank A., Medical devices and applications of polyhydroxyalkanoate polymers.
  18. Williams,Simon F.; Martin,David P.; Skraly,Frank A., Medical devices and applications of polyhydroxyalkanoate polymers.
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  20. Sherman, David H.; Williams, Mark D.; Xue, Yongquan, Metabolic engineering of polyhydroxyalkanoate monomer synthases.
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  30. Guillouet,Stephane; Sinskey,Anthony J.; Rodal,Avital A.; Lessard,Philip A., Methods for producing L-isoleucine.
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