[특허]Rapid thermal cycle apparatus

Rapid thermal cycle apparatus 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12M-001/40 C12M-001/38
출원번호	US-0166772 (1993-12-14)
발명자 / 주소	Haynes John L. (Chapel Hill NC)
출원인 / 주소	Becton, Dickinson and Company (Franklin Lakes NJ 02)
인용정보	피인용 횟수 : 62 인용 특허 : 0

초록 ▼

A thermal cycle apparatus comprises a body having a hollow interior and an access for the passage of liquid into an out of the body. Thermally conductive liquid is contained within the interior of the body. This liquid has a thermal capacity greater than the thermal capacity of the body itself. A pump or piston is provided for moving liquid into and out of the body in conjunction with the access opening. The liquid within the body is alternated between lower and higher temperatures in repeating cycles. A well or container for holding a sample of material to be subjected to cyclic thermal changes is held in contact with the liquid within the body in order to conduct the cyclic temperature changes of the liquid to the sample. A method for thermally cycling samples of material between lower and higher temperatures is also within the purview of the present invention.

대표청구항 ▼

A thermal cycle apparatus useful for the amplification of nucleic acid sequences comprising: a body having a hollow interior comprising an upper surface connected by first and second sidewalls and first and second endwalls to a lower surface wherein said sidewalls and said endwalls have a thickness of about 0.002 to about 0.125 inches (from about 0.051 to about 3.175 mm) and said body comprising a low thermal mass material selected from plastic or ceramic; a plurality of wells integrally formed with said upper surface of said body depending downwardly into said hollow interior; a first liquid passage port integrally formed with said first endwall; a second liquid passage port integrally formed with said second endwall; a first valve for directing fluid flow to and from said first liquid passage port; a second valve for directing fluid flow to and from said second liquid passage port; a first liquid tank and a first piston downstream from said first valve whereby a forward stroke of said first piston forces liquid from said first liquid tank through said first valve and onward into said body and a rearward stroke of said first piston forces liquid from said body through said first valve and onward into said first liquid tank; and a second liquid tank and a second piston downstream from said second valve whereby a forward stroke of said second piston forces liquid from said second liquid tank through said second valve and onward into said body and a rearward stroke of said second piston forces liquid from said body through said second valve and onward into said second liquid tank.

이 특허를 인용한 특허 (62)

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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Rapid thermal cycle apparatus 원문보기

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Rapid thermal cycle apparatus 원문보기

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