[특허]Method of measuring the luminescence emitted in a luminescent assay

Method of measuring the luminescence emitted in a luminescent assay 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/53
출원번호	US-0094085 (1993-07-28)
우선권정보	FR-0000930 (1991-01-28)
국제출원번호	PCT/FR92/00069 (1992-01-27)
§371/§102 date	19930728 (19930728)
국제공개번호	WO-9213264 (1992-08-06)
발명자 / 주소	Mabile Michel (La Celle Saint Cloud FRX) Mathis Grard (Bagnols Sur Ceze FRX) Jolu Etienne J.-P. (Bagnols Sur Ceze FRX) Pouyat Dominique (Roquemaure FRX) Dumont Christophe (Thourotte FRX)
출원인 / 주소	Cis Bio International (Saclay FRX 03)
인용정보	피인용 횟수 : 73 인용 특허 : 0

초록 ▼

The present invention relates to a method employing at least one luminescent tracer compound and a luminescent compound used as an internal reference, which, when exposed to the same excitation wavelength, are capable of emitting at difference wavelengths, l2 and l1 respectively, either by direct luminescence or by the induction of a luminescent emission, and correcting the measurement of the luminescence emitted by the tracer compound at wavelength l2 on the basis of the measurement of the luminescence emitted by the reference compound at wavelength l

대표청구항 ▼

A method for detecting or determining a target analyte in a sample in which it may be present by measurement of emitted luminescence in a chemiluminescent immunoassay, said method comprising the steps of: (a) forming a reaction mixture by contacting (i) the sample with (ii) a tracer compound comprising a receptor capable of specifically binding to the target analyte conjugated to a luminescent label capable of generating emitted luminescence at a measuring wavelength l2 in the presence of (iii) an internal reference compound capable of emitting luminescence at a correcting wavelength l1, in order to form a specific binding complex between the target analyte and the tracer, wherein the tracer compound and the internal reference compound are the same or different; (b) irradiating the reaction mixture at a single excitation wavelength by an external radiation source; (c) simultaneously measuring both the emitted luminescence at l2 due to the tracer bound in the specific binding complex and the emitted luminescence at l1 due to the internal reference compound which takes account of interference parameters of the reaction mixture; (d) calculating a corrected luminescence for the luminescence emitted by the tracer compound at l2 based on the luminescence emitted by the internal reference compound at l1; and (e) correlating the corrected luminescence reading to the presence or amount of the target analyte in the sample.

이 특허를 인용한 특허 (73)

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

내보내기 구분	파일저장 인쇄 메일전송
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연합인증

Method of measuring the luminescence emitted in a luminescent assay 원문보기

초록 ▼

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연합인증

Method of measuring the luminescence emitted in a luminescent assay 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

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이 특허를 인용한 특허 (73)

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