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|국가/구분||United States(US) Patent 등록|
|미국특허분류(USC)||435/6 ; 435/912 ; 935/77 ; 935/78|
|발명자 / 주소|
|출원인 / 주소|
|인용정보||피인용 횟수 : 4 인용 특허 : 3|
Methods for processing samples which may contain mycobacteria which are compatible with both conventional culturing techniques and nucleic acid analysis. It has been found that the harsh chemical treatment previously thought necessary to obtain efficient lysis can be eliminated without loss of lysis efficiency, thereby eliminating reagents which may inhibit subsequent nucleic acid-based reactions. Heat alone is sufficient to lyse mycobacteria, even in buffers which do not contain detergents, chelators, enzymes, etc. Residual reagents introduced by conven...
A method for processing Mycobacterium tuberculosis consisting essentially of: a) liquifying a sputum sample; b) centrifuging the liquified sample to form a liquified pellet; c) washing the liquified pellet two or three times with a wash solution consisting of saline, water or a buffer which does not contain detergents, enzymes or chelating agents, and centrifuging between washes at a speed sufficient to form a washed pellet which is resistant to loss of material during washing, and; d) resuspending the washed pellet in a solution consisting of saline, wa...