초록 ▼

A vessel and closure assembly for culturing cells wherein a gas permeable membrane is provided in the closure to allow rapid and uniform equilibration of gases between the atmosphere of the vessel and the atmosphere of the incubator. A plug is provided for occluding passage of gases through the gas

A vessel and closure assembly for culturing cells wherein a gas permeable membrane is provided in the closure to allow rapid and uniform equilibration of gases between the atmosphere of the vessel and the atmosphere of the incubator. A plug is provided for occluding passage of gases through the gas permeable membrane when the vessel is removed from the controlled atmosphere of the incubator.

대표청구항 ▼

A culture vessel assembly constructed to grow cell cultures in incubators comprising: a vessel comprising a chamber and a neck connected to said chamber having an opening for introducing cells and culture fluids into said chamber; a cap comprising a top portion, a bottom portion, an annular skirt ex

A culture vessel assembly constructed to grow cell cultures in incubators comprising: a vessel comprising a chamber and a neck connected to said chamber having an opening for introducing cells and culture fluids into said chamber; a cap comprising a top portion, a bottom portion, an annular skirt extending from said top portion to said bottom portion and having an inner surface and outer surface, an inner inverted skin portion surrounded by said inner surface of said annular skirt and extending from said top portion toward said bottom portion, an annular space between said inner surface of said annular skirt and said inverted skirt portion, a compartment area in said top portion as defined by said inverted skirt portion and an orifice in said top portion as defined by said inverted skirt portion surrounded by said compartment area, whereby said means for allowing gas diffusion into and out of said vessel is attached to said orifice; and a plug removably attached to said cap for occluding gas diffusion into and out of said vessel comprising a top flange integrally formed to a cylindrical base with lugs comprising a distal and proximal end with a cleat located at said proximal end and wherein said distal end extends underneath said top flange.