[특허]Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids

Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12Q-001/68 C12P-019/34 C12N-009/12 C12N-015/11
출원번호	US-0457647 (1995-06-01)
발명자 / 주소	Erlich Henry A. (Oakland CA) Horn Glenn (Emeryville CA) Saiki Randall K. (Richmond CA) Mullis Kary B. (Kensington CA)
출원인 / 주소	Hoffmann-La Roche Inc. (Nutley NJ 02)
인용정보	피인용 횟수 : 92 인용 특허 : 0

초록 ▼

Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.

대표청구항 ▼

A process for detecting the presence of a nucleotide variation in sequence in a nucleic acid contained in a sample, which process comprises: (a) treating the sample, together or sequentially, with four different nucleoside triphosphates, an agent for polymerization of the nucleoside triphosphates, and two oligonucleotide primers for said nucleic acid containing said variation under hybridizing conditions, such that a primer hybridizes to said nucleic acid and an extension product of the primer is synthesized which is complementary to said nucleotide variation in sequence, wherein said primers are selected such that the extension product synthesized from one primer, when separated from its complement, can serve as a template for synthesis of the extension product of the other primer; (b) treating the sample under denaturing conditions to separate the primer extension products from their templates; (c) treating the sample, together or sequentially, with said four nucleoside triphosphates, an agent for polymerization of the nucleoside triphosphates, and oligonucleotide primers such that a primer extension product is synthesized using each of the single strands produced in step (b) as a template, wherein steps (b) and (c) are repeated a sufficient number of times to result in detectable amplification of the nucleic acid containing the sequence variation; (d) directly transferring, without gel fractionation, the product of step (c) to a membrane; (e) treating the membrane under hybridization conditions with a labeled sequence-specific oligonucleotide probe between about 15 and about 25 nucleotides in length capable of hybridizing with the amplified nucleic acid sequence only if a sequence of the probe is complementary to a region of the amplified sequence; and (f) detecting whether the probe has hybridized to an amplified sequence in the nucleic acid sample.

이 특허를 인용한 특허 (92)

Shuber, Anthony P., Analysis of heterogeneous nucleic acid samples.
상세보기
Stephen P. A. Fodor ; Dennis W. Solas ; William J. Dower, Analysis of target molecules using an encoding system.
상세보기
Seul, Michael; Xia, Xiongwu; Chau, Chiu; Determan, Scott, Analysis, secure access to, and transmission of array images.
상세보기
Xia, Xiongwu; Seul, Michael; Chau, Chiu; Determan, Scott, Analysis, secure access to, and transmission of array images.
상세보기
Fodor, Stephen P. A.; Stryer, Lubert; Pirrung, Michael C.; Read, J. Leighton; Hoeprich, Jr., Paul D., Apparatus comprising polymers.
상세보기
Stryer, Luburt; Fodor, Stephen P. A.; Read, J. Leighton; Pirrung, Michael C., Apparatus for polymer synthesis.
상세보기
Banerjee, Sukanta; Seul, Michael; Li, Alice X., Arrays of magnetic particles.
상세보기
Xia, Xiongwu; Seul, Michael, Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification.
상세보기
Hashmi, Ghazala; Seul, Michael, Correcting an assay image of an array of signals generated from a multiplexed hybridization-mediated assay.
상세보기
Banerjee, Sukanta; Yang, Jiacheng; Seul, Michael, Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation.
상세보기
Hubert Koster ; Kai Tang ; Dong-Jing Fu ; Carsten W. Siegert DE; Daniel P. Little ; Andreas Braun ; Brigitte Darnhofer-Demar DE; Christian Jurinke DE; Dirk Van den Boom DE, DNA diagnostics based on mass spectrometry.
상세보기
K？ster,Hubert; Braun,Andreas; van den Boom,Dirk; Jurinke,Christian; Ruppert,Andreas; Hillenkamp,Franz; Little,Daniel P., DNA diagnostics based on mass spectrometry.
상세보기
K��ster,Hubert; Braun,Andreas; van den Boom,Dirk; Jurinke,Christian; Ruppert,Andreas; Hillenkamp,Franz, DNA diagnostics based on mass spectrometry.
상세보기
Koster Hubert, DNA sequencing by mass spectrometry via exonuclease degradation.
상세보기
Ho, Shiaolan Y.; Shah, Ankur H.; Chen, Xiaodi, Detection of single nucleotide polymorphisms in human KRAS.
상세보기
St. Clair Daret K. ; Urano Muneyasu,JPX ; Kasarskis Edward J., Diagnostic test and therapy for manganese superoxide dismutate (mNsod) associated diseases.
상세보기
Little Daniel P. ; Higgins Scott,GBX ; Koster Hubert, Diagnostics based on mass spectrometric detection of translated target polypeptides.
상세보기
Hashmi,Ghazala; Seul,Michael, Differentiating homozygous, heterozygous and wild-type alleles using a multiplexed hybridization-mediated assay.
상세보기
Singh, Rajinder; Ti, Leslie Low Eng; Li, Leslie Ooi Cheng; Abdullah, Meilina Ong; Nookiah, Rajanaidu; Sambanthamurthi, Ravigadevi; Smith, Steven W.; Lakey, Nathan D.; Martienssen, Rob; Ordway, Jared; Hogan, Michael, Gene controlling shell phenotype in palm.
상세보기
Wohlgemuth, Jay; Tayama, Darren; Walther, Dirk; Lal, Preeti G., Genes useful for diagnosing and monitoring inflammation related disorders.
상세보기
Hillenkamp, Franz, IR MALDI mass spectrometry of nucleic acids using liquid matrices.
상세보기
Hillenkamp, Franz, IR-MALDI mass spectrometry of nucleic acids using liquid matrices.
상세보기
O'Leary, Timothy J.; Mason, Jeffrey T., Immunoliposome-nucleic acid amplification (ILNAA) assay.
상세보기
O'Leary, Timothy J.; Mason, Jeffrey T., Immunoliposome-nucleic acid amplification (ILNAA) assay.
상세보기
Hillenkamp, Franz, Infrared matrix-assisted laser desorption/ionization mass spectrometric analysis of macromolecules.
상세보기
Richards, William L.; Ashby, Austin; Ketterer, Matthew; Marshall, Kevin; Harrison, Josh; Mette, Matthew; Richards, Paul; Showalter, Wayne; Cote, Jasmin; Halbert, Phillip C.; Bourgeois, Solene; Metzger, Steven W.; Hance, Ken; Mensack, Meghan; Michel, Carlos; Allers, Elke; Gamage, Dulini; Prisbrey, Landon; Gusyatin, Oleg; Shamsheyeva, Alena; Turng, Ben; Ghusson, Andrew; Reinhardt, Kurt, Instrument and system for rapid microogranism identification and antimicrobial agent susceptibility testing.
상세보기
Richards, William L.; Ashby, Austin; Ketterer, Matthew; Marshall, Kevin; Harrison, Josh; Mette, Matthew; Richards, Paul; Showalter, Wayne; Cote, Jasmin; Halbert, Phillip C.; Bourgeois, Solene; Metzger, Steven W.; Hance, Ken; Mensack, Meghan; Michel, Carlos; Allers, Elke; Gamage, Dulini; Prisbrey, Landon; Gusyatin, Oleg; Shamsheyeva, Alena; Turng, Ben; Ghusson, Andrew; Reinhardt, Kurt, Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing.
상세보기
Yang, Jiacheng, Kits for multiplexed nucleic acid analysis by capture of single-stranded DNA produced from double-stranded target fragments.
상세보기
Daniel Little ; Hubert Koster ; G. Scott Higgins GB; David Lough GB, Mass spectrometric detection of polypeptides.
상세보기
Little Daniel ; Koster Hubert ; Higgins G. Scott,GBX ; Lough David,GBX, Mass spectrometric detection of polypeptides.
상세보기
Köster, Hubert, Mass spectrometric methods for detecting mutations in a target nucleic acid.
상세보기
Kang Changwon,KRX ; Kwon Young-Soo,KRX ; Kim Young Tae,KRX ; Koster Hubert ; Little Daniel P. ; Little Maryanne J. ; Xiang Guobing ; Lough David M.,GBX ; Cantor Charles, Mass spectrometric methods for sequencing nucleic acids.
상세보기
Prisbrey, Landon; Blackmore, Elise; Hance, Kenneth R.; Metzger, Steven W.; Marshall, Kevin, Membrane-assisted purification.
상세보기
Shuber, Anthony P., Method for detecting a recombinant event.
상세보기
Shuber, Anthony P., Method for detecting recombinant event.
상세보기
Shuber, Anthony P., Method for monitoring disease progression or recurrence.
상세보기
Fodor, Stephen P. A.; Solas, Dennis W.; Dower, William J., Method of comparing nucleic acid sequences.
상세보기
Seul, Michael, Method of making a microbead array with attached biomolecules.
상세보기
Seul, Michael, Method of making a microbead array with attached biomolecules.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing and monitoring auto immune and chronic inflammatory diseases.
상세보기
Rosenberg, Steven; Fry, Kirk; Wu, Bin; Dedrick, Russell L., Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders.
상세보기
Wohlgemuth, Jay; Fry, Kirk E.; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, Macdonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing and monitoring transplant rejection.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, MacDonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, MacDonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, MacDonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
상세보기
Wohlgemuth,Jay; Fry,Kirk; Woodward,Robert; Ly,Ngoc, Methods and compositions for diagnosing and monitoring transplant rejection.
상세보기
Wohlgemuth,Jay; Fry,Kirk; Woodward,Robert; Ly,Ngoc; Prentice,James, Methods and compositions for diagnosing and monitoring transplant rejection.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases.
상세보기
Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases.
상세보기
Wohlgemuth, Jay; Fry, Kirk E.; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosis and monitoring auto immune and chronic inflammatory diseases.
상세보기
Landers, John; Jordan, Barbara; Housman, David E.; Charest, Alain, Methods and products related to genotyping and DNA analysis.
상세보기
Shuber, Anthony P.; Kann, Lisa; Whitney, Duncan, Methods for analysis of molecular events.
상세보기
Shuber, Anthony P.; Kann, Lisa; Whitney, Duncan, Methods for analysis of molecular events.
상세보기
Shalon, Tidhar Dari; Brown, Patrick O., Methods for constructing subarrays and subarrays made thereby.
상세보기
Sorge,Joseph A.; Whalen,Anne M., Methods for detection of a target nucleic acid by capture.
상세보기
Lal, Preeti G.; Williams, Gavin E.; Fry, Kirk E.; Sun, Jingtao; Dedrick, Russell L., Methods for diagnosing and monitoring the status of systemic lupus erythematosus.
상세보기
Stryer, Lubert; Fodor, Stephen P. A.; Read, J. Leighton; Pirrung, Michael C., Methods for polymer synthesis.
상세보기
Sorge, Joseph A., Methods of enriching for and identifying polymorphisms.
상세보기
Sorge, Joseph A., Methods of enriching for and identifying polymorphisms.
상세보기
Stryer, Lubert; Pirrung, Michael C.; Read, J. Leighton; Fodor, Stephen P. A., Methods of synthesizing a plurality of different polymers on a surface of a substrate.
상세보기
Wang, Xinwen; Banerjee, Sukanta, Microparticles with enhanced covalent binding capacity and their uses.
상세보기
Banerjee, Sukanta; Seul, Michael, Multianalyte molecular analysis using application-specific random particle arrays.
상세보기
Pirrung, Michael C.; Read, J. Leighton; Fodor, Stephen P. A.; Stryer, Lubert, Nucleic acid reading and analysis system.
상세보기
Stephen P. A. Fodor ; J. Leighton Read ; Lubert Stryer ; Michael C. Pirrung, Nucleic acid reading and analysis system.
상세보기
Ronaldus Adrianus Fouchier NL; Johanna Schuitemaker NL, Nucleic acids and methods for the discrimination between syncytium inducing and non syncytium inducing variants of the human immunodeficiency virus.
상세보기
Fodor,Stephen P. A.; Stryer,Lubert; Read,J. Leighton; Pirrung,Michael C., Nucleotides and analogs having photoremovable protecting groups.
상세보기
Seul, Michael; Chau, Chiu Wo; Tan, Enqing, Number coding for identification of subtypes of coded types of solid phase carriers.
상세보기
Seul, Michael; Banerjee, Sukanta; Yang, Jiacheng; Vener, Tatiana, Optimization of gene expression analysis using immobilized capture probes.
상세보기
Seul, Michael; Banerjee, Sukanta; Yang, Jiacheng; Vener, Tatiana, Optimization of gene expression analysis using immobilized capture probes.
상세보기
Michael C. Pirrung ; Lubert Stryer ; Stephen P. A. Fodor ; J. Leighton Read, Photolithographic and other means for manufacturing arrays.
상세보기
Stephen P. A. Fodor ; J. Leighton Read ; Lubert Stryer ; Michael C. Pirrung, Photolithographic and other means for manufacturing arrays.
상세보기
Fodor, Stephen P. A.; Read, J. Leighton; Stryer, Lubert; Pirrung, Michael C., Polypeptide arrays.
상세보기
Stephen P. A. Fodor ; Dennis W. Solas ; William J. Dower, Products for detecting nucleic acids.
상세보기
Metzger, Steven W.; Hance, Kenneth Robert, Rapid cell purification systems.
상세보기
Metzger, Steven W.; Hance, Kenneth Robert, Rapid cell purification systems.
상세보기
Metzger, Steven W.; Hance, Kenneth Robert, Rapid cell purification systems.
상세보기
Shamsheyeva, Alena; Howson, David C.; Metzger, Steven W., Rapid determination of microbial growth and antimicrobial susceptibility.
상세보기
Metzger, Steven W.; Howson, David C.; Goldberg, David A.; Buttry, Daniel A., Rapid microbial detection and antimicrobial susceptibility testing.
상세보기
West, Jason Andrew Appleton; Satterfield, Brent Coleman, Selection of aptamers based on geometry.
상세보기
Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
상세보기
Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
상세보기
Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
상세보기
Goldberg, David A.; Howson, David C.; Metzger, Steven W.; Buttry, Daniel A.; Saavedra, Steven Scott, Sensitive and rapid determination of antimicrobial susceptibility.
상세보기
Brown,Patrick O.; Shalon,Tidhar Dari, Substrates comprising polynucleotide microarrays.
상세보기
Fodor, Stephen P. A.; Pirrung, Michael C.; Stryer, Lubert; Read, J. Leighton, Support bound probes and methods of analysis using the same.
상세보기
J. Leighton Read ; Stephen P. A. Fodor ; Lubert Stryer ; Michael C. Pirrung, Support bound probes and methods of analysis using the same.
상세보기
Stephen P.A. Fodor ; James L. Winkler, System for determining receptor-ligand binding affinity.
상세보기
Mark L. Collins ; Donald N. Halbert ; Walter King ; Jonathan M. Lawrie, Target and background capture methods with amplification for affinity assays.
상세보기
Seul, Michael; Banerjee, Sukanta; Podual, Kairali; Hong, Ye, Un-supported polymeric film with embedded microbeads.
상세보기
Fodor, Stephen P. A.; Stryer, Lubert; Read, J. Leighton; Pirrung, Michael C., Very large scale immobilized polymer synthesis.
상세보기

IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 공고번호, 공고일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표출원인, 출원인국적, 출원인주소, 발명자, 발명자E, 발명자코드, 발명자주소, 발명자 우편번호, 발명자국적, 대표IPC, IPC코드, 요약, 미국특허분류, 대리인주소, 대리인코드, 대리인(한글), 대리인(영문), 국제공개일자, 국제공개번호, 국제출원일자, 국제출원번호, 우선권, 우선권주장일, 우선권국가, 우선권출원번호, 원출원일자, 원출원번호, 지정국, Citing Patents, Cited Patents
저장형식	Text(ASCII format) Excel format PIAS분석(.xls)
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

이 특허를 인용한 특허 (92)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

이 특허를 인용한 특허 (92)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트