초록 ▼

The invention relates to novel aromatic acids, especially to compounds of formula [Figure] (I) wherein R1 is lower alkyl, R2 is lower alkyl, R3 is hydrogen, carboxy or sulfo, R4 is carboxy or sulfo, G is an unsubstituted or substituted 1,4-phenylene group or an unsubstituted or substituted 1,4-napht

The invention relates to novel aromatic acids, especially to compounds of formula [Figure] (I) wherein R1 is lower alkyl, R2 is lower alkyl, R3 is hydrogen, carboxy or sulfo, R4 is carboxy or sulfo, G is an unsubstituted or substituted 1,4-phenylene group or an unsubstituted or substituted 1,4-naphthylene group, and wherein either R5 and R6 together are an additional bond and L is an oxygen or sulfur atom or wherein R5 is hydrogen, R6 is halomethyl and L is an oxygen atom, and salts thereof, to the use of compounds I and their salts, to a process for the preparation of compounds I and their salts, to starting materials used in that preparation process, and salts thereof, to a process for the preparation of those starting materials and their salts, to a device in which the compounds I and their salts are used, and to a process in which that device is used. The compounds of formula I can be used as adjuncts in the investigation of proteins and can be prepared in a manner known per se.

대표청구항 ▼

A device for modifying a protein with a chemical reagent and thereafter performing high-performance liquid chromatography (HPLC) analysis of the chemically modified protein, comprising a reactor; a vacuum pump connected to said reactor; syringe pump means for introducing the unmodified protein and c

A device for modifying a protein with a chemical reagent and thereafter performing high-performance liquid chromatography (HPLC) analysis of the chemically modified protein, comprising a reactor; a vacuum pump connected to said reactor; syringe pump means for introducing the unmodified protein and chemical reagent into the reactor for reaction, introducing inert gas into the reactor which, when kept at sufficiently high pressure in the reactor by the vacuum pump, causes evacuation of the reactor contents, and washing the reactor after evacuation by introduction of washing solution into the reactor; a desalting unit for separating excess reagents and by-products from chemically modified protein; a HPLC component for analyzing the reactor contents; a waste reservoir; control means for directing the reactor contents after evacuation from the reactor to the desalting unit for separation, to the HPLC component for analysis, or, if the reactor contents is washing solution, to the waste reservoir; an eluent delivery pump for delivering eluent to the desalting unit for use in the separating process; and a detector which detects whether the product exiting the desalting unit contains chemically modified protein, in which case the product is directed back into the reactor, or whether the product does not contain chemically modified protein, in which case the product is directed to the waste reservoir.