초록 ▼

An assay device for detection and/or determination of an analyte in a test sample uses a barrier containing an aperture to control the application of reagents to the device for greater reproducibility of results. In its simplest form, the device comprises: (1) a chromatographic medium having a first

An assay device for detection and/or determination of an analyte in a test sample uses a barrier containing an aperture to control the application of reagents to the device for greater reproducibility of results. In its simplest form, the device comprises: (1) a chromatographic medium having a first end, a second end, and first and second surfaces, and having a specific binding partner for the analyte immobilized thereto in a detection zone; (2) at least one absorber in operable contact with at least one of the first and second ends; and (3) a substantially fluid-impermeable barrier adjacent to the first surface of the chromatographic medium, the barrier having at least one aperture therethrough for application of liquid to the chromatographic medium, the barrier at least partially blocking application of liquid to the chromatographic medium. The device can be adapted for sandwich or competitive assays and can be used to perform amplified assays, such as those using silver amplification or enzyme amplification. Various arrangements of components within the device are possible, and elements such as filters can be accommodated.

대표청구항 ▼

A chromatographic assay device for detection and/or determination of an analyte in a test sample comprising: (a) a first opposable component including: (i) a chromatographic medium having a first end, a second end, and first and second surfaces, and having a specific binding partner for the analyte

A chromatographic assay device for detection and/or determination of an analyte in a test sample comprising: (a) a first opposable component including: (i) a chromatographic medium having a first end, a second end, and first and second surfaces, and having a specific binding partner for the analyte immobilized thereon in a detection zone between the first and second ends of the chromatographic medium; (ii) at least one absorber in operable contact with at least one of the first and second ends of the chromatographic medium; and (iii) a substantially fluid-impermeable barrier layered on top of the first surface of the chromatographic medium and having an aperture for application of liquid to the chromatographic medium, the barrier at least partially blocking application of liquid to the chromatographic medium; and (b) a second opposable component containing at least one reactant for applying the at least one reactant directly or indirectly to the chromatographic medium through the aperture; wherein the first and second opposable components are configured so that bringing the first and second opposable components into opposition results in the second opposable component applying the at least one reactant directly or indirectly to the chromatographic medium through the aperture.