초록 ▼

A process for carrying out a specific binding assay by reacting (a) a sample under assay for the possible presence of a substance being tested for, (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance

A process for carrying out a specific binding assay by reacting (a) a sample under assay for the possible presence of a substance being tested for, (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance being tested for which is conjugated to a detectable marker, to thereby form a complex by reacting between whatever quantities are present of the substance being tested for in (a) with reagents (b) and (c). The marker is immobilised to the support via the substance being tested for, and is detected or assayed as an index of the quantity present in the sample (a) of any of the substance. The reaction ingredients (a), (b) and (c) are all mixed in a single step for reaction in a single reaction liquid. Competitive interference between the binding reactions of the substance being tested for and reagents (b) and (c) is avoided by use of monoclonal antibodies of narrow and different, non-interfering specificity, reaction reagent (b) is immobilized on the surface of a displacer body.

대표청구항 ▼

In the process for carrying out a specific binding assay comprising the steps of reacting (a) a sample under assay, possibly containing a substance being tested for, with (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding par

In the process for carrying out a specific binding assay comprising the steps of reacting (a) a sample under assay, possibly containing a substance being tested for, with (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance being tested for which is conjugated to a detectable marker, thereby to form a sandwich complex by reaction between whatever quantities are present of the substance being tested for with reagents (b) and (c) and immobilising the marker to the support via the substance being tested for, the marker being detected or assayed as an index of the quantity of the substance being tested for present in the sample (a), the improvement which comprises using reagents (b) and (c) together for reaction with sample (a) and avoiding competitive interference between the binding reactions of the substance being tested for and reagents (b) and (c) by using as reagents (b) and (c), monoclonal antibodies each of narrow and different, non-interfering specificity, the binding reagent (b) being immobilised on the surface of a displacer body which occupies a majority of the volume of a well or cup containing aqueous liquid in which the specific binding reaction takes place.