초록 ▼

The present invention provides a much simplified method for the preparation of nucleic acid samples. This method provides many advantages over prior sample preparation methods in terms of greater sensitivity, reliability and ease of use. Importantly, the present invention provides a method in which

The present invention provides a much simplified method for the preparation of nucleic acid samples. This method provides many advantages over prior sample preparation methods in terms of greater sensitivity, reliability and ease of use. Importantly, the present invention provides a method in which nucleic acid samples may be prepared so as to conserve reagents and time. The samples so prepared are readily amplifiable and may be used for other purposes as well.

대표청구항 ▼

A method for preparing nucleic acid samples comprising, in sequential order, the steps of: a) providing in any order, i) a plasma sample suspected of containing ribonucleic acid, ii) an aqueous buffered solution consisting essentially of guanidinium thiocyanate, and beta-mercaptoethanol, iii) an alc

A method for preparing nucleic acid samples comprising, in sequential order, the steps of: a) providing in any order, i) a plasma sample suspected of containing ribonucleic acid, ii) an aqueous buffered solution consisting essentially of guanidinium thiocyanate, and beta-mercaptoethanol, iii) an alcohol, and iv) a reaction vessel; b) adding to said reaction vessel, in any order, said sample and said aqueous buffered solution, to make a reaction mixture; c) heating said reaction mixture to a temperature above 60°C.; d) adding said alcohol to precipitate said ribonucleic acid; and e) recovering said precipitated ribonucleic acid.