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[미국특허] Method for generating a subtracted cDNA library and uses of the generated library 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12P-019/34
출원번호 US-0143576 (1993-10-27)
발명자 / 주소
  • Fisher Paul B. (Scarsdale NY) Jiang Hongping (New York NY)
출원인 / 주소
  • The Trustees of Columbia University in the City of New York (New York NY 02)
인용정보 피인용 횟수 : 43  인용 특허 : 0

초록

This invention provides a method of generating a subtracted cDNA library of a cell comprising: a) generating a cDNA library of the cell; b) isolating double-stranded DNAs from the cDNA library; c) releasing the double-stranded cDNA inserts from the double-stranded DNAs; d) denaturing the isolated do

대표청구항

A method of generating a subtracted cDNA library of a population of cells comprising: a) generating a ll

이 특허를 인용한 특허 (43) 인용/피인용 타임라인 분석

  1. Onami,Shuichi; Hamahashi,Shugo; Miyano,Satoru; Kitano,Hiroaki, Cell lineage extracting method.
  2. Jackson, Merrill; Herman, Michael; Hoben, Grace; Sebastiao, Noemi; Cockayne, Scott; Ferrea, Heather, Compositions comprising nanomaterials and method for using such compositions for histochemical processes.
  3. Steven G. Reed ; Jiangchun Xu, Compounds for diagnosis of Breast cancer and methods for their use.
  4. Kim Lewis ; Peichun Hsieh, Culturing cells in presence of amphipathic weak bases and/or cations and multiple drug resistance inhibitor containing reserpine.
  5. Lewis, Kim; Hsieh, Peichun, Formulation containing amphipathic weak base and/or amphipathic cation and multiple drug resistance inhibitor.
  6. Kosmeder, Jerome W.; Lefever, Mark; Johnson, Donald; Farrell, Michael; Zhilina, Zhanna; Bieniarz, Christopher, Haptens, hapten conjugates, compositions thereof and method for their preparation and use.
  7. Kosmeder, Jerome W.; Lefever, Mark; Johnson, Donald; Farrell, Michael; Zhilina, Zhanna; Bieniarz, Christopher, Haptens, hapten conjugates, compositions thereof and method for their preparation and use.
  8. Kosmeder, II, Jerome W.; Lefever, Mark; Johnson, Donald; Farrell, Michael; Zhilina, Zhanna; Bieniarz, Christopher, Haptens, hapten conjugates, compositions thereof preparation and method for their preparation and use.
  9. Mandalam, Ramkumar; Xu, Chunhui; Gold, Joseph D.; Carpenter, Melissa K., Human embryonic stem cells genetically modified to contain a nucleic acid and cultured with fibroblast growth factor.
  10. Isola, Jorma; Tanner, Minna; Jarvinen, Tero; Shi, Zuo-Rong; Wu, Rina, Identifying subjects suitable for topoisomerase II inhibitor treatment.
  11. Fisher, Paul B.; Jiang, Hongping, MDA-7 nucleic acid molecules and pharmaceutical compositions thereof.
  12. Fisher,Paul B.; Jiang,Hongping, MDA-7 proteins and methods of use thereof.
  13. Mandalam,Ramkumar; Xu,Chunhui, Medium for growing human embryonic stem cells.
  14. Xu,Chunhui; Li,Yan; Mandalam,Ramkumar, Medium for growing human embryonic stem cells.
  15. Fisher,Paul B.; Kang,Dong Chul; Gopalkrishnan,Rahul V., Melanoma differentation associated gene-5 and vectors and cells containing same.
  16. Fisher,Paul B.; Kang,Dong Chul; Gopalkrishnan,Rahul V., Melanoma differentiation associated gene-5 and promoter and uses thereof.
  17. Fisher, Paul B.; Madireddi, Malavi T., Melanoma differentiation associated gene-7 promoter and uses thereof.
  18. Farrell, Michael, Method for producing nucleic acid probes.
  19. Farrell, Michael, Method for producing nucleic acid probes.
  20. Chen, Xiao-Bo; Bieniarz, Christopher; Farrell, Michael, Method for quantifying biomolecules conjugated to a nanoparticle.
  21. Farrell, Michael, Method for using permuted nucleic acid probes.
  22. Fisher, Paul B., Methods of treating various cancers using melanoma differentiation associated protein.
  23. Fisher, Paul B, Methods of treating various cancers using melanoma differentiation associated protein-7.
  24. Fisher,Paul B, Methods of treating various cancers using melanoma differentiation associated protein-7.
  25. Mandalam, Ramkumar; Xu, Chunhui; Gold, Joseph D.; Carpenter, Melissa K., Micro-carrier culture system for rapid expansion of human embryonic stem cells.
  26. Wu-Bo Li ; Paul E. Nisson ; Joel Jessee, Normalized nucleic acid libraries and methods of production thereof.
  27. Wang Xun ; Duvick Jonathan P. ; Briggs Steven P., PCR-based cDNA substractive cloning method.
  28. Wang Xun ; Duvick Jonathan P. ; Briggs Steven P., PCR-based cDNA subtractive cloning method.
  29. Kosmeder, II, Jerry W.; Kernag, Casey A.; Johnson, Donald; Bieniarz, Christopher, Polymeric carriers for immunohistochemistry and in situ hybridization.
  30. Kosmeder, II, Jerry W.; Kernag, Casey A.; Johnson, Donald; Bieniarz, Christopher, Polymeric carriers for immunohistochemistry and in situ hybridization.
  31. Kosmeder, II, Jerry W.; Kernag, Casey A.; Johnson, Donald; Bieniarz, Christopher, Polymeric carriers for immunohistochemistry and in situ hybridization.
  32. Kosmeder, Jerome W.; Kernag, Casey A.; Johnson, Donald; Bieniarz, Christopher, Polymeric carriers for immunohistochemistry and in situ hybridization.
  33. Kosmeder, Jerry W.; Kernag, Casey A.; Johnson, Donald; Bieniarz, Christopher, Polymeric carriers for immunohistochemistry and in situ hybridization.
  34. Kosmeder, Jerry W.; Kernag, Casey A.; Johnson, Donald; Bieniarz, Christopher, Polymeric carriers for immunohistochemistry and in situ hybridization.
  35. Christians, Fred C.; Do, Duc; Gingeras, Thomas; Gunderson, Kevin; Miyada, Charles G.; Rosenow, Carsten; Wu, Kai; Yang, Qing, Preparation of nucleic acid samples.
  36. Mandalam, Ramkumar; Xu, Chunhui; Gold, Joseph D.; Carpenter, Melissa K., Primate pluripotent stem cell expansion without feeder cells and in the presence of FGF and matrigel or Engelbreth-Holm-Swarm tumor cell preparation.
  37. Mandalam, Ramkumar; Xu, Chunhui; Gold, Joseph D.; Carpenter, Melissa K., Screening methods for human embryonic stem cells.
  38. Chada, Sunil, Use of MDA-7 to inhibit pathogenic infectious organisms.
  39. Paul B. Fisher, Use of a melanoma differentiation associated gene (mda-7) for inducing apoptosis of a tumor cell.
  40. Carpenter,Melissa K.; Inokuma,Margaret S.; Xu,Chunhui, Use of human embryonic stem cells for drug screening and toxicity testing.
  41. Fisher Paul B. ; Jiang Hongping, Uses of mda-6.
  42. Funk, Walter D.; Carpenter, Melissa K.; Gold, Joseph D.; Inokuma, Margaret S.; Xu, Chunhui, cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells.
  43. Fisher,Paul B., mda-9 protein.

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