초록

This invention provides highly thermally stable trehalose phosphorylases. The trehalose phosphorylases of the present invention can be obtained from cultured mixture of microorganisms and are highly thermally stable, and produce aaa

대표청구항 ▼

An isolated trehalose phosphorylase enzyme produced by a microorganism selected from the group consisting of Grifola, Pleurotus, Lyophyllum, Lentinus, Agaricus, Trametes, Coriolus, Lenzites, Schizophyllum, Panus, Crepidotus, Laetiporus, Polyporellus, Favolus, Trichaptum, Oudemansiella, Naematoloma,

An isolated trehalose phosphorylase enzyme produced by a microorganism selected from the group consisting of Grifola, Pleurotus, Lyophyllum, Lentinus, Agaricus, Trametes, Coriolus, Lenzites, Schizophyllum, Panus, Crepidotus, Laetiporus, Polyporellus, Favolus, Trichaptum, Oudemansiella, Naematoloma, Rhodophyllus, Gloeophyllum, Fomes, Ganoderma, Elfvingia, Fomitopsis, Armillariella, Lampteromyces, Pholiota and Tricholoma genera, which enzyme catalyzes the reaction of trehalose with inorganic phosphoric acid or salt thereof to produce a1-phosphate and D-glucose, and the reaction of a1-phosphate with D-glucose to produce trehalose and an inorganic phosphoric acid, and wherein the enzyme has a molecular weight of about 120,000 daltons as determined by gel filtration and about 60,000 daltons as determined by SDS-PAGE.