초록 ▼

The invention relates to an anti-inflammatory factor isolated from milk, methods of purifying the anti-inflammatory factor resulting in substantially or highly purified preparations and to methods for using this factor to remove adhered neutrophils from endothelial cells, to prevent the emigration o

The invention relates to an anti-inflammatory factor isolated from milk, methods of purifying the anti-inflammatory factor resulting in substantially or highly purified preparations and to methods for using this factor to remove adhered neutrophils from endothelial cells, to prevent the emigration of cells from the vasculature and to suppress the response of lymphocytes to foreign antigens.

대표청구항 ▼

A method for purifying an anti-inflammatory factor from skimmed milk comprising: (i) ultrafiltering said milk through a filter with a molecular weight cut-off of 10,000 daltons; (ii) collecting a 10,000 dalton permeate from step (i); (iii) applying said permeate to a first ion exchange column and wa

A method for purifying an anti-inflammatory factor from skimmed milk comprising: (i) ultrafiltering said milk through a filter with a molecular weight cut-off of 10,000 daltons; (ii) collecting a 10,000 dalton permeate from step (i); (iii) applying said permeate to a first ion exchange column and washing said column with deionized water; (iv) collecting the eluate from step (iii); (v) separating the eluate from step (iv) on a preparative size exclusion HPLC chromatography column and collecting the eluate; and (vi) performing organic partition extraction of the eluate obtained from said preparative size exclusion HPLC chromatography and obtaining the aqueous extract from said extraction.