초록 ▼

The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits

The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.

대표청구항 ▼

A method for detecting an analyte in a sample, comprising the steps of: contacting a sample containing a target analyte with a labeled binding component to specifically bind the target analyte and form a labeled binding component-target complex, wherein the labeled binding component comprises a bind

A method for detecting an analyte in a sample, comprising the steps of: contacting a sample containing a target analyte with a labeled binding component to specifically bind the target analyte and form a labeled binding component-target complex, wherein the labeled binding component comprises a binding component attached to an up-converting inorganic phosphor particle comprising at least one rare earth element and a phosphor host material and being capable of converting excitation radiation to emission radiation of a shorter wavelength; separating any unbound labeled binding component from the labeled binding component-target complex; illuminating the labeled binding component-target complex with excitation radiation; and detecting emission radiation of at least one label emission wavelength, wherein the emission radiation has a shorter wavelength than the excitation radiation.