초록 ▼

The present invention comprises a microtiter plate formed of a substantially rigid, polymeric plate having a substantially flat upper surface and a regular array of similar wells, typically either cylindrical or frusto-conical, each well being defined by a fluid-impervious peripheral wall extending

The present invention comprises a microtiter plate formed of a substantially rigid, polymeric plate having a substantially flat upper surface and a regular array of similar wells, typically either cylindrical or frusto-conical, each well being defined by a fluid-impervious peripheral wall extending a predetermined distance along an axis substantially perpendicularly to that upper surface between an opening in the upper surface and a well bottom. Disposed within the well adjacent the bottom is a porous structure providing a surface area at least five times greater than the surface area of the interior well bottom. The well bottom may be either fluid impervious or pervious. Where the well bottom is fluid pervious, it may be formed from a fluid impervious sheet apertured to accept and be bonded to the peripheries of the ends of a plurality of fluid pervious ultrafiltration fibers that may have hollow cores. In embodiments with fluid pervious well bottoms, a vacuum plenum is provided below the wells for drawing fluid from the wells through the pervious material. In embodiments in which the well bottom is fluid impervious, the porous structure within the well and coupled to the inner surface of the well bottom can be formed from either continuous or closed cells, a plurality of loops of fibers having both ends coupled to the bottom, a plurality of fibers having one end coupled to the bottom, a coil of fiber, and other configurations.

대표청구항 ▼

In a microtiter plate comprising a substantially rigid plate having at least one substantially flat surface and at least one well therein, said well being defined by an opening in said surface, a substantially fluid-impervious barrier forming a bottom of said well and spaced apart from and extending

In a microtiter plate comprising a substantially rigid plate having at least one substantially flat surface and at least one well therein, said well being defined by an opening in said surface, a substantially fluid-impervious barrier forming a bottom of said well and spaced apart from and extending substantially parallel to said surface, and a fluid-impervious peripheral wall extending a predetermined distance along an axis substantially perpendicularly to said surface between said opening in said surface and said well bottom, the improvement comprising means bonded to said well bottom and extending, at least in part, upwardly within said well from said well bottom toward said flat surface, for defining a surface area substantially greater than the cross-sectional area of said well adjacent said well bottom and orthogonal to said axis.