Immune reactivity to HER-2/neu protein for diagnosis and treatment of malignancies in which the HER-2/neu oncogene is a
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/536
G01N-033/541
G01N-033/566
C07K-016/30
출원번호
US-0467083
(1995-06-06)
발명자
/ 주소
Cheever Martin A.
Disis Mary L.
출원인 / 주소
University of Washington
대리인 / 주소
Seed and Berry LLP
인용정보
피인용 횟수 :
86인용 특허 :
9
초록▼
Methods for the detection, monitoring and treatment of malignancies in which the HER-2/neu oncogene is associated are disclosed. Detection of specific T cell activation (e.g., by measuring the proliferation of T cells) in response to in vitro exposure to the HER-2/neu protein, or detection of immuno
Methods for the detection, monitoring and treatment of malignancies in which the HER-2/neu oncogene is associated are disclosed. Detection of specific T cell activation (e.g., by measuring the proliferation of T cells) in response to in vitro exposure to the HER-2/neu protein, or detection of immunocomplexes formed between the HER-2/neu protein and antibodies in body fluid, allows the diagnosis of the presence of a malignancy in which the HER-2/neu oncogene is associated. The present invention also discloses methods and compositions, including peptides, for treating such malignancies.
대표청구항▼
[ We claim:] [1.] A method of screening for the presence of a malignancy in a warm-blooded animal, wherein a HER-2/neu oncogene is associated with the malignancy, comprising the steps of:(a) contacting a body fluid, suspected of containing antibodies specific for HER-2/neu protein, with HER-2/neu pr
[ We claim:] [1.] A method of screening for the presence of a malignancy in a warm-blooded animal, wherein a HER-2/neu oncogene is associated with the malignancy, comprising the steps of:(a) contacting a body fluid, suspected of containing antibodies specific for HER-2/neu protein, with HER-2/neu protein;(b) incubating the body fluid under conditions and for a time sufficient to allow immunocomplexes to form; and(c) detecting the presence or absence of immunocomplexes formed between the HER-2/neu protein and antibodies in the body fluid specific for the HER-2/neu protein, thereby determining the presence or absence of the malignancy. [11.] A method for detecting changes in the level of antibodies specific for HER-2/neu protein in a human, comprising the steps of:(a) contacting a first body fluid sample with HER-2/neu protein;(b) incubating the sample under conditions and for a time sufficient to allow immunocomplexes to form;(c) detecting immunocomplexes formed between the HER-2/neu protein and antibodies in the sample that are specific for the HER-2/neu protein;(d) repeating steps (a), (b) and (c) on a second body fluid sample taken from the same individual at a time subsequent to the first sample; and(e) comparing the number of immunocomplexes detected in the first and second body fluid samples, thereby determining whether a change in the level of antibody specific for HER-2/neu protein has occurred. The method of claim 11 wherein a HER-2/neu protein is associated with a malignancy selected from the group consisting of breast, ovarian, colon, lung and prostate cancer. The method of claim 11 wherein a reporter group is bound to a second antibody capable of binding to the antibodies, and wherein the step of detecting comprises (a) removing substantially any unbound antibody, (b) adding the second antibody, (c) removing substantially any unbound second antibody, and (d) detecting the presence or absence of the reporter group. The method of claim 13 wherein the second antibody is an anti-human antibody. The method of claim 13 wherein the reporter group is selected from the group consisting of radioisotopes, fluorophores, enzymes, luminescers, and dye particles. The method of claim 11 wherein a reporter group is bound to a molecule capable of binding to the immunocomplexes, and wherein the step of detecting comprises (a) adding the molecule, (b) removing substantially any unbound molecule, and (c) detecting the presence or absence of the reporter group. The method of claim 16 wherein the molecule capable of binding to the immunocomplexes is protein A. The method of claim 16 wherein the reporter group is selected from the group consisting of radioisotopes, fluorophores, enzymes, luminescers, and dye particles. The method of claim 11 wherein a reporter group is bound to the HER-2/neu protein, and wherein the step of detecting comprises removing substantially any unbound HER-2/neu protein and thereafter detecting the presence or absence of the reporter group.
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