초록 ▼

Edible fruit, seed and vegetables of transgenic plants modified to produce a sweetening protein such as monellin or thaumatin are useful in preparing food compositions which have enhanced sweetness improved flavor. Expression systems for the genes encoding sweetening proteins compatible with plant s

Edible fruit, seed and vegetables of transgenic plants modified to produce a sweetening protein such as monellin or thaumatin are useful in preparing food compositions which have enhanced sweetness improved flavor. Expression systems for the genes encoding sweetening proteins compatible with plant systems and designed to enhance the production of these proteins in the edible portions of plants, and methods for producing sweetened fruit, seeds and vegetables are described.

대표청구항 ▼

[ We claim:] [1.] A recombinant expression system capable, when inserted into a higher plant, of expressing the gene encoding a single-chain monellin, which system comprises a nucleotide sequence encoding said single-chain monellin operably linked to control sequences effective in said higher plant,

[ We claim:] [1.] A recombinant expression system capable, when inserted into a higher plant, of expressing the gene encoding a single-chain monellin, which system comprises a nucleotide sequence encoding said single-chain monellin operably linked to control sequences effective in said higher plant,wherein said single-chain monellin is of the formula B-C-A;wherein B represents a peptide portion at least 90% homologous to residues 1-46 of the B chain of native monellin depicted in SEQ ID NO:2 and modified only by conservative substitutions;C is a covalent bond or a peptide portion of 1-10 gene-encoded amino acids; andA represents a peptide portion at least 90% homologous to residues 6-45 of the A chain of native monellin depicted in SEQ ID NO: 1 and modified only by conservative substitution.